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YWHAZ (基因名), 14-3-3 protein zeta/delta (蛋白名), 1433Z_BOVIN.

产品名称:

Bovine YWHAZ/ 14-3-3 protein zeta/delta ELISA Kit
14-3-3蛋白ζ/δ

货号:

E14294b

商标:

EIAab^®

监管等级:

RUO

别名:

Factor activating exoenzyme S, Protein kinase C inhibitor protein 1, FAS, KCIP-1

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

78-5000pg/mL

灵敏度:

40.5pg/mL

特异性:

Natural and recombinant bovine 14-3-3 protein zeta/delta

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

Neurosciences

规格 & 价格:

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产品说明书

说明书:

MSDS:

在线询价

数据
文献
序列 / 3D
常见问题解答
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通用注释

亚单元:

Homodimer. Heterodimerizes with YWHAE (By similarity). Homo- and hetero-dimerization is inhibited by phosphorylation on Ser-58 (By similarity). Interacts with FOXO4, NOXA1, SSH1 ARHGEF2, CDK16 and BSPRY. Interacts with WEE1 (C-terminal). Interacts with MLF1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with BAX; the interaction occurs in the cytoplasm. Under stress conditions, MAPK8-mediated phosphorylation releases BAX to mitochondria. Interacts with TP53; the interaction enhances p53 transcriptional activity. The Ser-58 phosphorylated form inhibits this interaction and p53 transcriptional activity. Interacts with ABL1 (phosphorylated form); the interaction retains ABL1 in the cytoplasm. Interacts with PKA-phosphorylated AANAT; the interaction modulates AANAT enzymatic activity by increasing affinity for arylalkylamines and acetyl-CoA and protecting the enzyme from dephosphorylation and proteasomal degradation. It may also prevent thiol-dependent inactivation (By similarity). Interacts with AKT1; the interaction phosphorylates YWHAZ and modulates dimerization (By similarity). Interacts with GAB2. Interacts with BCL2L11, SAMSN1 and TLK2 (By similarity). Interacts with phosphorylated RAF1; the interaction is inhibited when YWHAZ is phosphorylated on Thr-232. Interacts with Thr-phosphorylated ITGB2. Interacts with the 'Thr-369' phosphorylated form of DAPK2 (By similarity). Interacts with PI4KB, TBC1D22A and TBC1D22B (By similarity). Interacts with ZFP36L1 (via phosphorylated form); this interaction occurs in a p38 MAPK- and AKT-signaling pathways (By similarity). Interacts with SLITRK1.

功能:

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Activates the ADP-ribosyltransferase (exoS) activity of bacterial origin.

亚细胞位置:

Cytoplasm Melanosome Located to stage I to stage IV melanosomes.

数据库链接

UniGene:

Bt.111451

SMR:

P63103

STRING:

9913.ENSBTAP00000000289

KEGG:

bta:287022

Pfam:

PF00244

Uniprot:

P63103

相关产品

Human YWHAZ ELISA Kit

Mouse Ywhaz ELISA Kit

Rat Ywhaz ELISA Kit

Chicken YWHAZ ELISA Kit

Human YWHAZ CLIA Kit

序列长度:

245

序列:

仅供参考，详细请咨询（services@eiaab.com）

50:

100:

150:

200:

245:

AELDTLSEES | YKDSTLIMQL | LRDNLTLWTS | DTQGDEAEAG | EGGEN

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

牛14-3-3蛋白ζ/δ(YWHAZ)ELISA试剂盒可以做多少个样本？

牛14-3-3蛋白ζ/δ(YWHAZ)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

牛14-3-3蛋白ζ/δ(YWHAZ)ELISA试剂盒使用视频？

牛14-3-3蛋白ζ/δ(YWHAZ)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

牛14-3-3蛋白ζ/δ(YWHAZ)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的牛14-3-3蛋白ζ/δ(YWHAZ)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

牛14-3-3蛋白ζ/δ(YWHAZ)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

牛14-3-3蛋白ζ/δ(YWHAZ)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析牛14-3-3蛋白ζ/δ(YWHAZ)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

牛14-3-3蛋白ζ/δ(YWHAZ)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集牛14-3-3蛋白ζ/δ(YWHAZ)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

牛14-3-3蛋白ζ/δ(YWHAZ)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

牛14-3-3蛋白ζ/δ(YWHAZ)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

牛14-3-3蛋白ζ/δ(YWHAZ)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Bovine YWHAZ/ 14-3-3 protein zeta/delta ELISA Kit 14-3-3蛋白ζ/δ

E14294b

Factor activating exoenzyme S, Protein kinase C inhibitor protein 1, FAS, KCIP-1

Bovine YWHAZ/ 14-3-3 protein zeta/delta ELISA Kit
14-3-3蛋白ζ/δ