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YWHAQ (GeneName), 14-3-3 protein theta (ProteinName), 1433T_HUMAN.
Product Name:

Human YWHAQ/ 14-3-3 protein theta ELISA Kit

 
Cat.#:

E14293h

Brand:
EIAab®
Regulatory Status:
Alternative:

14-3-3 protein T-cell,14-3-3 protein tau,Protein HS1

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.78-50ng/mL
Sensitivity:
0.43ng/ml
Specificity:
Natural and recombinant human 14-3-3 protein theta
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Cancer
Product Overview:
E14293h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (14-3-3 protein theta) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 14-3-3 protein theta. The ELISA analytical biochemical technique of the E14293h kit is based on 14-3-3 protein theta antibody-14-3-3 protein theta antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 14-3-3 protein theta antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, 14-3-3 protein theta. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human YWHAQ ELISA Kit
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Product Datasheets
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.8%

Recovery
Recovery was determined by spiking various levels of 14-3-3 protein theta into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

88

82-94

Plasma

90

84-96

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 14-3-3 protein theta and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

101-111%

87-99%

109-121%

91-103%

EDTA plasma(n=5)

107-115%

115-125%

92-102%

106-115%

heparin plasma(n=5)

95-105%

 

108-116%

95-104%

110-119%

 

General Annotation


Sub Unit:
Homodimer. Interacts with CDK16 (By similarity). Interacts with RGS7 (phosphorylated form) (PubMed:10862767). Interacts with SSH1. Interacts with CDKN1B ('Thr-198' phosphorylated form); the interaction translocates CDKN1B to the cytoplasm. Interacts with GAB2. Interacts with the 'Ser-241' phosphorylated form of PDPK1. Interacts with the 'Thr-369' phosphorylated form of DAPK2 (PubMed:26047703). Interacts with PI4KB, TBC1D22A and TBC1D22B (PubMed:23572552). Interacts with SLITRK1 (PubMed:19640509).


Function:
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.


Subcellular Location:
Cytoplasm In neurons, axonally transported to the nerve terminals.


This product has not yet been referenced specifically in any publications.
Publishing research using this product ?
Please let us know and we will provide a credit for your next order.

[1].
"A 14-3-3 mRNA is up-regulated in amyotrophic lateral sclerosis spinal cord."

[2].
"An inflammatory bowel disease-risk variant in INAVA decreases pattern recognition receptor-induced outcomes."

[3].
"Front-signal-dependent accumulation of the RHOA inhibitor FAM65B at leading edges polarizes neutrophils."

[4].
"ACBD3 interaction with TBC1 domain 22 protein is differentially affected by enteroviral and kobuviral 3A protein binding."

[5].
"Evaluating new candidate SNPs as low penetrance risk factors in sporadic breast cancer: a two-stage Spanish case-control study."

[6].
"SLITRK1 binds 14-3-3 and regulates neurite outgrowth in a phosphorylation-dependent manner."

[7].
"Phosphorylation-dependent binding of 14-3-3 terminates signalling by the Gab2 docking protein."

[8].
"Structural basis for protein-protein interactions in the 14-3-3 protein family."

[9].
"A pathway of neuregulin-induced activation of cofilin-phosphatase Slingshot and cofilin in lamellipodia."

[10].
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Sample Data
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