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YWHAG (GeneName), 14-3-3 protein gamma (ProteinName), 1433G_HUMAN.

Product Name:

Human YWHAG/ 14-3-3 protein gamma ELISA Kit

Cat.#:

Brand:

EIAab^®

Regulatory Status:

RUO

Alternative:

Protein kinase C inhibitor protein 1,KCIP-1

Detection Method:

ELISA

Specificity:

Natural and recombinant human 14-3-3 protein gamma

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Research Area:

Cancer

Product Overview:

is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (14-3-3 protein gamma) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 14-3-3 protein gamma. The ELISA analytical biochemical technique of the kit is based on 14-3-3 protein gamma antibody-14-3-3 protein gamma antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 14-3-3 protein gamma antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, 14-3-3 protein gamma. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

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Human YWHAG ELISA Kit

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Data
Citations
Publication
Sequence / 3D
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General Annotation

Sub Unit:

Homodimer. Interacts with SAMSN1 (By similarity). Interacts with RAF1, SSH1 and CRTC2/TORC2. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with GAB2. Interacts with MDM4 (phosphorylated); negatively regulates MDM4 activity toward TP53. Interacts with PKA-phosphorylated AANAT and SIRT2.Interacts with the 'Thr-369' phosphorylated form of DAPK2 (PubMed:26047703). Interacts with PI4KB, TBC1D22A and TBC1D22B (PubMed:23572552). Interacts with SLITRK1 (PubMed:19640509).

Function:

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Subcellular Location:

Cytoplasm

Database links

UniGene:

Hs.744840

SMR:

P61981

STRING:

9606.ENSP00000306330

KEGG:

hsa:7532

MIM:

605356

Pfam:

PF00244

Uniprot:

P61981

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Sequence length:

247

Sequence:

50:

100:

150:

200:

247:

FDDAIAELDT | LNEDSYKDST | LIMQLLRDNL | TLWTSDQQDD | DGGEGNN

3D Structure:

Predicted Eptitope:

Vaild Sequence:

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[1].

"Cloning, expression, and chromosomal mapping of the human 14-3-3gamma gene (YWHAG) to 7q11.23."

(PMID: 10486217)

[2].

"14-3-3Gamma interacts with and is phosphorylated by multiple protein kinase C isoforms in PDGF-stimulated human vascular smooth muscle cells."

(PMID: 10433554)

[3].

"De Novo Mutations in YWHAG Cause Early-Onset Epilepsy."

(PMID: 28777935)

[4].

"ACBD3 interaction with TBC1 domain 22 protein is differentially affected by enteroviral and kobuviral 3A protein binding."

(PMID: 23572552)