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YWHAB (GeneName), 14-3-3 protein beta/alpha (ProteinName), 1433B_BOVIN.
Product Name:

Bovine YWHAB/ 14-3-3 protein beta/alpha ELISA Kit

Regulatory Status:

Protein kinase C inhibitor protein 1,KCIP-1

Detection Method:
Natural and recombinant bovine 14-3-3 protein beta/alpha
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Research Area:
Product Overview:
is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (14-3-3 protein beta/alpha) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 14-3-3 protein beta/alpha. The ELISA analytical biochemical technique of the kit is based on 14-3-3 protein beta/alpha antibody-14-3-3 protein beta/alpha antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 14-3-3 protein beta/alpha antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, 14-3-3 protein beta/alpha. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Datasheets

General Annotation

Sub Unit:
Homodimer (By similarity). Interacts with SAMSN1 and PRKCE (By similarity). Interacts with AKAP13. Interacts with SSH1 and TORC2/CRTC2. Interacts with ABL1; the interaction results in cytoplasmic location of ABL1 and inhibition of cABL-mediated apoptosis. Interacts with ROR2 (dimer); the interaction results in phosphorylation of YWHAB on tyrosine residues. Interacts with GAB2. Interacts with YAP1 (phosphorylated form). Interacts with the phosphorylated (by AKT1) form of SRPK2. Interacts with PKA-phosphorylated AANAT. Interacts with MYO1C. Interacts with SIRT2 (By similarity). Interacts with the 'Thr-369' phosphorylated form of DAPK2 (By similarity). Interacts with PI4KB, TBC1D22A and TBC1D22B. Interacts with the 'Ser-1134' and 'Ser-1161' phosphorylated form of SOS1 (By similarity). Interacts (via phosphorylated form) with YWHAB; this interaction occurs in a protein kinase AKT1-dependent manner (By similarity). Interacts with SLITRK1. Interacts with SYNPO2 (phosphorylated form); YWHAB competes with ACTN2 for interaction with SYNPO2.

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.

Subcellular Location:
Cytoplasm Melanosome

This product has not yet been referenced specifically in any publications.
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"Front-signal-dependent accumulation of the RHOA inhibitor FAM65B at leading edges polarizes neutrophils."

"Crystal structure of human myosin 1c--the motor in GLUT4 exocytosis: implications for Ca2+ regulation and 14-3-3 binding."

"ACBD3 interaction with TBC1 domain 22 protein is differentially affected by enteroviral and kobuviral 3A protein binding."

"A proteomic perspective of inbuilt viral protein regulation: pUL46 tegument protein is targeted for degradation by ICP0 during herpes simplex virus type 1 infection."

"A-kinase anchoring protein (AKAP)-Lbc anchors a PKN-based signaling complex involved in α1-adrenergic receptor-induced p38 activation."

"Interaction of Akt-phosphorylated SRPK2 with 14-3-3 mediates cell cycle and cell death in neurons."

"SLITRK1 binds 14-3-3 and regulates neurite outgrowth in a phosphorylation-dependent manner."

"Phosphorylation-dependent binding of 14-3-3 terminates signalling by the Gab2 docking protein."

"Examination of association to autism of common genetic variationin genes related to dopamine."

"Inactivation of YAP oncoprotein by the Hippo pathway is involved in cell contact inhibition and tissue growth control."
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