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GABA (短名 ),

产品名称:

General GABA/ Gamma-Aminobutyric acid ELISA Kit
γ氨基丁酸

货号:

E0900Ge

商标:

EIAab^®

监管等级:

RUO

检测方法:

ELISA

实验类型:

Competitive

检测范围:

1.56-100ng/mL

灵敏度:

0.45ng/mL

特异性:

Natural and recombinant general Gamma-Aminobutyric acid

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

规格 & 价格:

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产品说明书

数据表:

说明书:

MSDS:

数据
文献 ( 13 )
序列 / 3D
常见问题解答
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线性

线性：给定样本通过梯度稀释，每次稀释的测量值与理论值的比值。

Sample	1：2	1:4	1:8	1:16
serum(n=5)	104-114%	83-93%	94-103%	86-96%
EDTA plasma(n=5)	102-113%	81-93%	102-112%	90-102%
heparin plasma(n=5)	106-116%	80-93%	92-101%	96-104%

通用注释

亚单元:

N/A

功能:

γ-Aminobutyric acid is the chief inhibitory neurotransmitter in the mammalian central nervous system. It plays a role in regulating neuronal excitability throughout the nervous system. In humans, GABA is also directly responsible for the regulation of muscle tone. Although chemically it is an amino acid, GABA is rarely referred to as such in the scientific or medical communities, because the term "amino acid," used without a qualifier, conventionally refers to the alpha amino acids, which GABA is not, nor is it ever incorporated into a protein. In spastic diplegia in humans, GABA absorption becomes impaired by nerves damaged from the condition's upper motor neuron lesion, which leads to hypertonia of the muscles signaled by those nerves that can no longer absorb GABA.

Location:

N/A

相关产品

General GABA CLIA Kit

序列长度:

N/A

序列:

N/A

3D 模型:

3D 结构:

免疫原序列:

验证序列:

[1].

"Angiopoietin-like protein 8/betatrophin as a new determinant of type 2 diabetes remission after bariatric surgery"

Miriam E, Marianne B, Nuria V, et al.

From: Translational Research

[2].

"Programming of a developmental imbalance in hypothalamic glutamatergic/GABAergic afferents mediates low basal activity of the hypothalamic-pituitary-adrenal axis induced by prenatal dexamethasone exposure in male offspring rats"

J Lu, Q Li, D Xu, et al.

From: Toxicology Letters

[3].

"Melatonin exerts a neuroprotective effect against γ-radiation-induced brain injury in the rat through the modulation of neurotransmitters, inflammatory cytokines, oxidative stress, and apoptosis"

M A El-Missiry, S Shabana, S J. Ghazala, et al.

From: Environmental Science and Pollution Research

[4].

"Neurotoxic effects of silver nanoparticles and the protective role of rutin"

Mona M. A, Mohamed M.A. H.

From: Biomedicine & Pharmacotherapy

[5].

"Topiramate mitigates 3-nitropropionic acid-induced striatal neurotoxicity via modulation of AMPA receptors"

H N. Shalaby, D M. EI-Tanbouly, H F. Zaki.

From: Food and Chemical Toxicology

[6].

"Protective Efficacy of Spirulina platensis Against Cadmium Induced Neurotoxicity in Rats"

RTM Alam, MY Hendawi

From: Global Veterinaria

[7].

"Therapeutic potential of endothelial progenitor cells in a rat model of epilepsy: Role of autophagy"

N N. Shahin, M M. Safar, S M. Rizk, et al.

From: Journal of Advanced Research

[8].

"Bone Marrow-Derived Endothelial Progenitor Cells Protect Against Scopolamine-Induced Alzheimer-Like Pathological Aberrations"

Safar M M, Arab H H, Rizk S M, et al.

From: Molecular neurobiology

[9].

"Comparative protective effects of royal jelly and cod liver oil against neurotoxic impact of tartrazine on male rat pups brain"

Mohamed A A R, Galal A A A, Elewa Y H A.

From: Acta histochemica

[10].

"The role of apitoxin in alleviating propionic acid-induced neurobehavioral impairments in rat pups: The expression pattern of Reelin gene"

Maha H. D, Manar E. S, Yasmina M. A, et al.

From: Biomedicine & Pharmacotherapy

[11].

"Amelioration of fenitrothion induced oxidative DNA damage and inactivation of caspase-3 in the brain and spleen tissues of male rats by N-acetylcysteine"

R T. Alam, T S. Imam, A H Arisha, et al.

From: Life Sciences

[12].

"Nanoformulated ellagic acid ameliorates pentylenetetrazol-induced experimental epileptic seizures by modulating oxidative stress, inflammatory cytokines and apoptosis in the brains of male mice"

M A. El-Missiry, A I. Othman, M A. Amer, et al.

From: Metabolic Brain Disease

[13].

"Prenatal ethanol exposure-induced hypothalamic an imbalance of glutamatergic/GABAergic projections and low functional expression in male offspring rats"

J Lu, Q Li, GQ Ma, et al.

From: Food and Chemical Toxicology

[1].

通用γ氨基丁酸(GABA)ELISA试剂盒可以做多少个样本？

通用γ氨基丁酸(GABA)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

通用γ氨基丁酸(GABA)ELISA试剂盒使用视频？

通用γ氨基丁酸(GABA)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

通用γ氨基丁酸(GABA)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的通用γ氨基丁酸(GABA)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

通用γ氨基丁酸(GABA)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

通用γ氨基丁酸(GABA)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析通用γ氨基丁酸(GABA)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

通用γ氨基丁酸(GABA)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集通用γ氨基丁酸(GABA)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

通用γ氨基丁酸(GABA)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

通用γ氨基丁酸(GABA)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

通用γ氨基丁酸(GABA)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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General GABA/ Gamma-Aminobutyric acid ELISA Kit γ氨基丁酸

E0900Ge

General GABA/ Gamma-Aminobutyric acid ELISA Kit
γ氨基丁酸