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Home  >  ELISA Kit  >  Rat OxLDL ELISA Kit
OxLDL (Shorten Name),
Product Name:

Rat OxLDL/ Oxidized low-density lipoprotein ELISA Kit

 
Cat.#:

E0527r

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.47-30ng/mL
Sensitivity:
0.237ng/mL
Specificity:
Natural and recombinant rat Oxidized low-density lipoprotein
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0527r is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Oxidized low-density lipoprotein) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Oxidized low-density lipoprotein. The ELISA analytical biochemical technique of the E0527r kit is based on Oxidized low-density lipoprotein antibody-Oxidized low-density lipoprotein antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Oxidized low-density lipoprotein antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Oxidized low-density lipoprotein. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Rat OxLDL ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.3%

Recovery
Recovery was determined by spiking various levels of Oxidized low-density lipoprotein into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

101

95-107

Plasma

103

97-109

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Oxidized low-density lipoprotein and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

83-93%

104-114%

100-112%

90-100%

EDTA plasma(n=5)

86-95%

104-114%

106-115%

96-107%

heparin plasma(n=5)

93-102%

 

84-94%

101-111%

104-115%

 

General Annotation


Sub Unit:
N/A


Function:
LDL particles pose a risk for cardiovascular disease when they invade the endothelium and becomes oxidized, since the oxidized forms are more easily retained by the proteoglycans. A complex set of biochemical reactions regulates the oxidation of LDL particles, chiefly stimulated by presence of necrotic cell debries and free radicals in the endothelium.


Location:
N/A


Sample Data
Sample Data
Sample Data
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