OxLDL (Shorten Name ),
Product Name:
Rat OxLDL/ Oxidized low-density lipoprotein ELISA Kit
Cat.#:
E0527r
Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.47-30ng/mL
Sensitivity:
0.237ng/mL
Specificity:
Natural and recombinant rat Oxidized low-density lipoprotein
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:

Research Area:
-
- Data
- Citations ( 1 )
- Sequence / 3D
- Feedback Wall (0)
Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤5.8%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤10.3%
Recovery
Recovery was determined by spiking various levels of Oxidized low-density lipoprotein into serum and plasma.
Sample Type |
Average(%) |
Recovery Range(%) |
Serum |
101 |
95-107 |
Plasma |
103 |
97-109 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Oxidized low-density lipoprotein and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
serum(n=5) |
83-93% |
104-114% |
100-112% |
90-100% |
EDTA plasma(n=5) |
86-95% |
104-114% |
106-115% |
96-107% |
heparin plasma(n=5) |
93-102%
|
84-94% |
101-111% |
104-115% |
General Annotation
Sub Unit:
N/A
Function:
LDL particles pose a risk for cardiovascular disease when they invade the endothelium and becomes oxidized, since the oxidized forms are more easily retained by the proteoglycans. A complex set of biochemical reactions regulates the oxidation of LDL particles, chiefly stimulated by presence of necrotic cell debries and free radicals in the endothelium.
Location:
N/A
[1].
Qing Li, Yi Wang, Ke Chen, Qing Zhou, Wei Wei, Yiping Wang, Yuan Wang
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