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OxLDL (Shorten Name),

Product Name:

Rat OxLDL/ Oxidized low-density lipoprotein ELISA Kit

Cat.#:

E0527r

Brand:

EIAab^®

Regulatory Status:

RUO

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.47-30ng/mL

Sensitivity:

0.237ng/mL

Specificity:

Natural and recombinant rat Oxidized low-density lipoprotein

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Product Overview:

E0527r is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Oxidized low-density lipoprotein) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Oxidized low-density lipoprotein. The ELISA analytical biochemical technique of the E0527r kit is based on Oxidized low-density lipoprotein antibody-Oxidized low-density lipoprotein antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Oxidized low-density lipoprotein antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Oxidized low-density lipoprotein. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

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Rat OxLDL ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations ( 1 )
Sequence / 3D
Feedback Wall (0)

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.3%

Recovery

Recovery was determined by spiking various levels of Oxidized low-density lipoprotein into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	101	95-107
Plasma	103	97-109

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Oxidized low-density lipoprotein and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	83-93%	104-114%	100-112%	90-100%
EDTA plasma(n=5)	86-95%	104-114%	106-115%	96-107%
heparin plasma(n=5)	93-102%	84-94%	101-111%	104-115%

General Annotation

Sub Unit:

N/A

Function:

LDL particles pose a risk for cardiovascular disease when they invade the endothelium and becomes oxidized, since the oxidized forms are more easily retained by the proteoglycans. A complex set of biochemical reactions regulates the oxidation of LDL particles, chiefly stimulated by presence of necrotic cell debries and free radicals in the endothelium.

Location:

N/A

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