CTXII (Shorten Name),
Product Name:
Rat CTXII/ C-telopeptide of Collagen alpha-1(II) chain ELISA Kit
Cat.#:
E0686r
Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
78-5000pg/ml
Sensitivity:
39pg/mL
Specificity:
Natural and recombinant rat C-telopeptide of Collagen alpha-1(II) chain
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0686r is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay)
Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (C-telopeptide of Collagen alpha-1(II) chain) ELISA Kit target analytes in biological samples. The concentration gradients of
the kit standards or positive controls render a theoretical kit detection range in biological research samples containing C-telopeptide of Collagen alpha-1(II) chain. The ELISA analytical biochemical
technique of the E0686r kit is based on C-telopeptide of Collagen alpha-1(II) chain antibody-C-telopeptide of Collagen alpha-1(II) chain antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C-telopeptide of Collagen alpha-1(II) chain
antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, C-telopeptide of Collagen alpha-1(II) chain. Appropriate sample types may include undiluted human body fluids
and/or tissue homogenates,
secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤6.5%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤9.3%
Recovery
Recovery was determined by spiking various levels of C-telopeptide of Collagen alpha-1(II) chain into serum and plasma.
|
Sample Type |
Average(%) |
Recovery Range(%) |
|
Serum |
101 |
95-107 |
|
Plasma |
103 |
97-109 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C-telopeptide of Collagen alpha-1(II) chain and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1：2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
109-119% |
110-120% |
90-100% |
94-103% |
|
EDTA plasma(n=5) |
92-102% |
103-114% |
98-107% |
102-110% |
|
heparin plasma(n=5) |
107-116%
|
100-110% |
95-105% |
110-120% |
General Annotation
Sub Unit:
N/A
Function:
In bone physiology, the C-terminal telopeptide (or more formally, carboxy-terminal collagen crosslinks, and known by the acronym CTX) is a serum biomarker used to measure the rate of bone turnover. It can be useful in assisting clinicians to determine a patient's nonsurgical treatment response as well as evaluate a patient's risk of developing complications during healing following surgical intervention. The test used to detect the CTX marker is called the Serum CrossLaps, and it is more specific to bone resorption than any other test currently available.
Location:
N/A
Vaild Sequence:
This product has not yet been referenced specifically in any publications.
Publishing research using this product ?
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Publishing research using this product ?
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