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5METHF (Shorten Name),
Product Name:

General 5METHF/ Levomefolic acid ELISA Kit

 
Cat.#:

E15141Ge

Brand:
EIAab®
Regulatory Status:
Alternative:

5-Methyltetrahydrofolate

Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.156-10ng/mL
Sensitivity:
0.078ng/mL
Specificity:
Natural and recombinant general Levomefolic acid
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E15141Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Levomefolic acid) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Levomefolic acid. The ELISA analytical biochemical technique of the E15141Ge kit is based on Levomefolic acid antibody-Levomefolic acid antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Levomefolic acid antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Levomefolic acid. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General 5METHF ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Levomefolic acid and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

107-116%

101-112%

104-114%

108-120%

EDTA plasma(n=5)

106-106%

82-93%

108-117%

93-105%

heparin plasma(n=5)

110-120%

 

105-117%

84-93%

107-116%

 

General Annotation


Sub Unit:
N/A


Function:
Levomefolic acid (also known as 5-MTHF, L-methylfolate and 5-methyltetrahydrofolate and (6S)-5-methyltetrahydrofolate, and (6S)-5-MTHF) is the primary biologically active form of folic acid used at the cellular level for DNA reproduction, the cysteine cycle and the regulation of homocysteine. It is also the form found in circulation and transported across membranes into tissues and across the blood-brain barrier. In the cell, L-methylfolate is used in the methylation of homocysteine to form methionine and tetrahydrofolate (THF). THF is the immediate acceptor of one carbon units for the synthesis of thymidine-DNA, purines (RNA and DNA) and methionine. The un-methylated form, folic acid (vitamin B9), is a synthetic form of folate, and must undergo enzymatic reduction by methylenetetrahydrofolate reductase (MTHFR) to become biologically active.


Location:
N/A


This product has not yet been referenced specifically in any publications.
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