General 5METHF/ Levomefolic acid ELISA Kit

Cat.#:

E15141Ge

Brand:

EIAab^®

Regulatory Status:

RUO

Alternative:

5-Methyltetrahydrofolate

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

0.156-10ng/mL

Sensitivity:

0.078ng/mL

Specificity:

Natural and recombinant general Levomefolic acid

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

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General 5METHF ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations
Sequence / 3D
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Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Levomefolic acid and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	107-116%	101-112%	104-114%	108-120%
EDTA plasma(n=5)	106-106%	82-93%	108-117%	93-105%
heparin plasma(n=5)	110-120%	105-117%	84-93%	107-116%

General Annotation

Sub Unit:

N/A

Function:

Levomefolic acid (also known as 5-MTHF, L-methylfolate and 5-methyltetrahydrofolate and (6S)-5-methyltetrahydrofolate, and (6S)-5-MTHF) is the primary biologically active form of folic acid used at the cellular level for DNA reproduction, the cysteine cycle and the regulation of homocysteine. It is also the form found in circulation and transported across membranes into tissues and across the blood-brain barrier. In the cell, L-methylfolate is used in the methylation of homocysteine to form methionine and tetrahydrofolate (THF). THF is the immediate acceptor of one carbon units for the synthesis of thymidine-DNA, purines (RNA and DNA) and methionine. The un-methylated form, folic acid (vitamin B9), is a synthetic form of folate, and must undergo enzymatic reduction by methylenetetrahydrofolate reductase (MTHFR) to become biologically active.

Location:

N/A

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