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8ISOPG (Shorten Name),
Product Name:

Human 8ISOPG/ 8-iso-Prostaglandin F2α ELISA Kit

 
Cat.#:

E0793h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
15.6-1000pg/mL
Sensitivity:
10pg/mL
Specificity:
Natural and recombinant human 8-iso-Prostaglandin F2α
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0793h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (8-iso-Prostaglandin F2α) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 8-iso-Prostaglandin F2α. The ELISA analytical biochemical technique of the E0793h kit is based on 8-iso-Prostaglandin F2α antibody-8-iso-Prostaglandin F2α antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 8-iso-Prostaglandin F2α antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, 8-iso-Prostaglandin F2α. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human 8ISOPG ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.2%

Recovery
Recovery was determined by spiking various levels of 8-iso-Prostaglandin F2α into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

102

96-108

Plasma

104

98-110

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 8-iso-Prostaglandin F2α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

98-107%

113-123%

107-120%

81-93%

EDTA plasma(n=5)

97-108%

91-101%

98-109%

80-90%

heparin plasma(n=5)

107-115%

 

98-98%

89-99%

101-111%

 

General Annotation


Sub Unit:
N/A


Function:
Prostaglandin F2α (PGF2α in prostanoid nomenclature), pharmaceutically termed dinoprost (INN), is a naturally occurring prostaglandin used in medicine to induce labor and as an abortifacient. In domestic mammals, it is produced by the uterus when stimulated by oxytocin, in the event that there has been no implantation during the follicular phase. It acts on the corpus luteum to cause luteolysis, forming a corpus albicans and stopping the production of progesterone. Action of PGF2α is dependent on the number of receptors on the corpus luteum membrane. The PGF2α isoform 8-iso-PGF2α was found in significantly increased amounts in patients with endometriosis, thus being a potential causative link in endometriosis-associated oxidative stress.


Location:
N/A


This product has not yet been referenced specifically in any publications.
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