General CdiGMP/ Cyclic Diguanylate ELISA Kit

Cat.#:

E0152Ge

Brand:

EIAab^®

Regulatory Status:

RUO

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

0.78-50ng/mL

Sensitivity:

0.22ng/mL

Specificity:

Natural and recombinant general Cyclic Diguanylate

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Product Overview:

E0152Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Cyclic Diguanylate) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Cyclic Diguanylate. The ELISA analytical biochemical technique of the E0152Ge kit is based on Cyclic Diguanylate antibody-Cyclic Diguanylate antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Cyclic Diguanylate antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Cyclic Diguanylate. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

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General CdiGMP ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations ( 3 )
Sequence / 3D
Feedback Wall (0)

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cyclic Diguanylate and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	88-101%	115-115%	105-115%	101-113%
EDTA plasma(n=5)	95-105%	83-93%	81-91%	85-96%
heparin plasma(n=5)	87-97%	90-103%	102-111%	92-102%

General Annotation

Sub Unit:

N/A

Function:

Cyclic di-GMP (also called cyclic diguanylate and c-di-GMP) is a second messenger used in signal transduction in a wide variety of bacteria. Cyclic di-GMP is not known to be used by eukaryotes or archaea. The biological role of cyclic di-GMP was first uncovered when it was identified as an allosteric activator of a cellulose synthase found in Gluconacetobacter xylinus.

Location:

N/A

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