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ACH (Shorten Name),
Product Name:

General ACH/ Acetylcholine ELISA Kit

 
Cat.#:

E0912Ge

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
78-5000pg/mL
Sensitivity:
24pg/mL
Specificity:
Natural and recombinant general Acetylcholine
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0912Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Acetylcholine) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Acetylcholine. The ELISA analytical biochemical technique of the E0912Ge kit is based on Acetylcholine antibody-Acetylcholine antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Acetylcholine antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Acetylcholine. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General ACH ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Acetylcholine and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

101-109%

94-103%

98-108%

95-108%

EDTA plasma(n=5)

96-106%

87-96%

98-107%

105-114%

heparin plasma(n=5)

105-118%

 

82-93%

97-107%

100-109%

 

General Annotation


Sub Unit:
N/A


Function:
Acetylcholine has functions both in the peripheral nervous system (PNS) and in the central nervous system (CNS) as a neuromodulator. Its receptors have very high binding constants. In the peripheral nervous system, acetylcholine activates muscles, and is a major neurotransmitter in the autonomic nervous system. In the central nervous system, acetylcholine and the associated neurons form a neurotransmitter system, the cholinergic system, which tends to cause anti-excitatory actions.


Location:
N/A


This product has not yet been referenced specifically in any publications.
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