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IgM (Shorten Name ),
Product Name:

Mouse IgM/ Immunoglobulin M ELISA Kit

Cat.#:

E0543m

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
7.8-500ng/mL
Sensitivity:
3.91ng/mL
Specificity:
Natural and recombinant mouse Immunoglobulin M
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Immunology
Mouse IgM ELISA Kit
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Mouse IgM ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.4%

Recovery
Recovery was determined by spiking various levels of Immunoglobulin M into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

81

80-87

Plasma

83

80-89

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Immunoglobulin M and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

82-94%

103-113%

108-118%

97-106%

EDTA plasma(n=5)

106-115%

105-115%

106-115%

94-107%

heparin plasma(n=5)

97-105%

 

90-100%

89-99%

110-122%

 

General Annotation


Sub Unit:
N/A


Function:
Due to its polymeric nature, IgM possesses high avidity, and is particularly effective at complement activation. By itself, IgM is an ineffective opsonin; however it contributes greatly to opsonization by activating complement and causing C3b to bind to the antigen.


Location:
Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to antigen.


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