Home > ELISA Kit > Mouse IgM ELISA Kit

IgM (Shorten Name),

Product Name:

Mouse IgM/ Immunoglobulin M ELISA Kit

Cat.#:

E0543m

Brand:

EIAab^®

Regulatory Status:

RUO

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

7.8-500ng/mL

Sensitivity:

3.91ng/mL

Specificity:

Natural and recombinant mouse Immunoglobulin M

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Immunology

Product Overview:

E0543m is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Immunoglobulin M) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Immunoglobulin M. The ELISA analytical biochemical technique of the E0543m kit is based on Immunoglobulin M antibody-Immunoglobulin M antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Immunoglobulin M antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Immunoglobulin M. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

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Mouse IgM ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations ( 3 )
Sequence / 3D
Feedback Wall (0)

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.4%

Recovery

Recovery was determined by spiking various levels of Immunoglobulin M into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	81	80-87
Plasma	83	80-89

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Immunoglobulin M and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	82-94%	103-113%	108-118%	97-106%
EDTA plasma(n=5)	106-115%	105-115%	106-115%	94-107%
heparin plasma(n=5)	97-105%	90-100%	89-99%	110-122%

General Annotation

Sub Unit:

N/A

Function:

Due to its polymeric nature, IgM possesses high avidity, and is particularly effective at complement activation. By itself, IgM is an ineffective opsonin; however it contributes greatly to opsonization by activating complement and causing C3b to bind to the antigen.

Location:

Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to antigen.

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