IgM (Shorten Name),
Product Name:
Mouse IgM/ Immunoglobulin M ELISA Kit
Cat.#:
E0543m
Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
7.8-500ng/mL
Sensitivity:
3.91ng/mL
Specificity:
Natural and recombinant mouse Immunoglobulin M
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Immunology
Product Overview:
E0543m is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay)
Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Immunoglobulin M) ELISA Kit target analytes in biological samples. The concentration gradients of
the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Immunoglobulin M. The ELISA analytical biochemical
technique of the E0543m kit is based on Immunoglobulin M antibody-Immunoglobulin M antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Immunoglobulin M
antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Immunoglobulin M. Appropriate sample types may include undiluted human body fluids
and/or tissue homogenates,
secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤6.8%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤9.4%
Recovery
Recovery was determined by spiking various levels of Immunoglobulin M into serum and plasma.
|
Sample Type |
Average(%) |
Recovery Range(%) |
|
Serum |
81 |
80-87 |
|
Plasma |
83 |
80-89 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Immunoglobulin M and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1：2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
82-94% |
103-113% |
108-118% |
97-106% |
|
EDTA plasma(n=5) |
106-115% |
105-115% |
106-115% |
94-107% |
|
heparin plasma(n=5) |
97-105%
|
90-100% |
89-99% |
110-122% |
General Annotation
Sub Unit:
N/A
Function:
Due to its polymeric nature, IgM possesses high avidity, and is particularly effective at complement activation. By itself, IgM is an ineffective opsonin; however it contributes greatly to opsonization by activating complement and causing C3b to bind to the antigen.
Location:
Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to antigen.
Vaild Sequence:
[1].
[2].
MS AL-Harbi, RZ Hamza, AA Dwary.
[3].
El-Shenawy N S, AL-Harbi M S, Hamza R Z.
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