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NAG (Shorten Name),
Product Name:

Human NAG/ N-acetyl-beta-D-glucosaminidase ELISA Kit

 
Cat.#:

E0069h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
1.56-100U/L
Sensitivity:
0.78U/L
Specificity:
Natural and recombinant human N-acetyl-beta-D-glucosaminidase
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0069h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (N-acetyl-beta-D-glucosaminidase) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing N-acetyl-beta-D-glucosaminidase. The ELISA analytical biochemical technique of the E0069h kit is based on N-acetyl-beta-D-glucosaminidase antibody-N-acetyl-beta-D-glucosaminidase antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect N-acetyl-beta-D-glucosaminidase antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, N-acetyl-beta-D-glucosaminidase. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human NAG ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

Recovery
Recovery was determined by spiking various levels of N-acetyl-beta-D-glucosaminidase into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

96

90-102

Plasma

98

92-104

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of N-acetyl-beta-D-glucosaminidase and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

105-113%

102-112%

103-112%

103-112%

EDTA plasma(n=5)

101-111%

90-100%

82-92%

94-104%

heparin plasma(n=5)

98-109%

 

95-106%

112-124%

105-113%

 

General Annotation


Sub Unit:
N/A


Function:
N/A


Location:
N/A


This product has not yet been referenced specifically in any publications.
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