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BEP (Shorten Name),
Product Name:

Mouse BEP/ Beta-endorphin ELISA Kit

 
Cat.#:

E0806m

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
15.6-1000pg/mL
Sensitivity:
8.12pg/mL
Specificity:
Natural and recombinant mouse Beta-endorphin
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0806m is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Beta-endorphin) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Beta-endorphin. The ELISA analytical biochemical technique of the E0806m kit is based on Beta-endorphin antibody-Beta-endorphin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Beta-endorphin antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Beta-endorphin. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Mouse BEP ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.1%

Recovery
Recovery was determined by spiking various levels of Beta-endorphin into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

86

80-92

Plasma

88

82-94

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Beta-endorphin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

104-116%

101-110%

102-114%

113-123%

EDTA plasma(n=5)

81-93%

102-111%

90-102%

86-97%

heparin plasma(n=5)

103-113%

 

92-101%

98-109%

92-92%

 

General Annotation


Sub Unit:
N/A


Function:
N/A


Location:
N/A


Sample Data
Sample Data
Sample Data
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