BEP (Shorten Name),
Product Name:
Mouse BEP/ Beta-endorphin ELISA Kit
Cat.#:
E0806m
Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
15.6-1000pg/mL
Sensitivity:
8.12pg/mL
Specificity:
Natural and recombinant mouse Beta-endorphin
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0806m is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay)
Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Beta-endorphin) ELISA Kit target analytes in biological samples. The concentration gradients of
the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Beta-endorphin. The ELISA analytical biochemical
technique of the E0806m kit is based on Beta-endorphin antibody-Beta-endorphin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Beta-endorphin
antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Beta-endorphin. Appropriate sample types may include undiluted human body fluids
and/or tissue homogenates,
secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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- Data
- Citations ( 1 )
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤6.8%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤8.1%
Recovery
Recovery was determined by spiking various levels of Beta-endorphin into serum and plasma.
Sample Type | Average(%) | Recovery Range(%) |
Serum | 86 | 80-92 |
Plasma | 88 | 82-94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Beta-endorphin and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1：2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 104-116% | 101-110% | 102-114% | 113-123% |
EDTA plasma(n=5) | 81-93% | 102-111% | 90-102% | 86-97% |
heparin plasma(n=5) | 103-113%
| 92-101% | 98-109% | 92-92% |
General Annotation
Sub Unit:
N/A
Function:
N/A
Location:
N/A
Vaild Sequence:
[1].
S Sasaki, Y Tamaki, K Nagata, et al.
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