Mouse BEP/ Beta-endorphin ELISA Kit

Cat.#:

E0806m

Brand:

EIAab^®

Regulatory Status:

RUO

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

15.6-1000pg/mL

Sensitivity:

8.12pg/mL

Specificity:

Natural and recombinant mouse Beta-endorphin

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Product Overview:

E0806m is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Beta-endorphin) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Beta-endorphin. The ELISA analytical biochemical technique of the E0806m kit is based on Beta-endorphin antibody-Beta-endorphin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Beta-endorphin antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Beta-endorphin. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

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Mouse BEP ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
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Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.1%

Recovery

Recovery was determined by spiking various levels of Beta-endorphin into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	86	80-92
Plasma	88	82-94

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Beta-endorphin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	104-116%	101-110%	102-114%	113-123%
EDTA plasma(n=5)	81-93%	102-111%	90-102%	86-97%
heparin plasma(n=5)	103-113%	92-101%	98-109%	92-92%