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SAM (Shorten Name ),
Product Name:

General SAM/ S-Adenosyl methionine ELISA Kit

Cat.#:

E14920Ge

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
1.56-100nmol/L
Sensitivity:
0.38nmol/L
Specificity:
Natural and recombinant general S-Adenosyl methionine
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
General SAM ELISA Kit
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General SAM ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of S-Adenosyl methionine and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

97-107%

85-97%

102-111%

101-111%

EDTA plasma(n=5)

92-102%

82-92%

81-90%

105-117%

heparin plasma(n=5)

102-112%

 

82-94%

100-110%

98-108%

 

General Annotation


Sub Unit:
N/A


Function:
S-Adenosyl methionine (ademetionine, AdoMet, SAM, SAMe, SAM-e) is a common cosubstrate involved in methyl group transfers. SAM was first discovered in Italy by G. L. Cantoni in 1952. It is made from adenosine triphosphate (ATP) and methionine by methionine adenosyltransferase (EC 2.5.1.6). Transmethylation, transsulfuration, and aminopropylation are the metabolic pathways that use SAM. Although these anabolic reactions occur throughout the body, most SAM is produced and consumed in the liver. The methyl group (CH3) attached to the methionine sulfur atom in SAM is chemically reactive. This allows donation of this group to an acceptor substrate in transmethylation reactions. More than 40 metabolic reactions involve the transfer of a methyl group from SAM to various substrates, such as nucleic acids, proteins, lipids and secondary metabolites. In bacteria, SAM is bound by the SAM riboswitch, which regulates genes involved in methionine or cysteine biosynthesis.


Location:
N/A


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