027-59234612(+86)
EIAab
Home  >  ELISA Kit  >  General SAM ELISA Kit
SAM (Shorten Name),
Product Name:

General SAM/ S-Adenosyl methionine ELISA Kit

 
Cat.#:

E14920Ge

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
1.56-100nmol/L
Sensitivity:
0.38nmol/L
Specificity:
Natural and recombinant general S-Adenosyl methionine
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E14920Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (S-Adenosyl methionine) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing S-Adenosyl methionine. The ELISA analytical biochemical technique of the E14920Ge kit is based on S-Adenosyl methionine antibody-S-Adenosyl methionine antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect S-Adenosyl methionine antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, S-Adenosyl methionine. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
More
General SAM ELISA Kit
Package & Price: Sign in.
Attention Attention
×
General SAM ELISA Kit
Email *
Message *
Please Sign in.
Product Datasheets
Datasheet:
Instruction:
MSDS:


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of S-Adenosyl methionine and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

97-107%

85-97%

102-111%

101-111%

EDTA plasma(n=5)

92-102%

82-92%

81-90%

105-117%

heparin plasma(n=5)

102-112%

 

82-94%

100-110%

98-108%

 

General Annotation


Sub Unit:
N/A


Function:
S-Adenosyl methionine (ademetionine, AdoMet, SAM, SAMe, SAM-e) is a common cosubstrate involved in methyl group transfers. SAM was first discovered in Italy by G. L. Cantoni in 1952. It is made from adenosine triphosphate (ATP) and methionine by methionine adenosyltransferase (EC 2.5.1.6). Transmethylation, transsulfuration, and aminopropylation are the metabolic pathways that use SAM. Although these anabolic reactions occur throughout the body, most SAM is produced and consumed in the liver. The methyl group (CH3) attached to the methionine sulfur atom in SAM is chemically reactive. This allows donation of this group to an acceptor substrate in transmethylation reactions. More than 40 metabolic reactions involve the transfer of a methyl group from SAM to various substrates, such as nucleic acids, proteins, lipids and secondary metabolites. In bacteria, SAM is bound by the SAM riboswitch, which regulates genes involved in methionine or cysteine biosynthesis.


Location:
N/A


Sample Data
Sample Data
Sample Data
My Cart close
Attention close
History close
Clear All
Comparison close
User
My Cart
Attention
History
Comparison
Top
Message
close_message
Product Name: *
Email: *
Message: *
Inform
new Message
Package Quantity Price ($) Subtotal 1 ($)
Subtotal 2: $
triangle
Package Quantity Price ($)
Do you want to be our agent and get a lower discount?
Please contact us:
Tel:027-59234612(+86)
Fax:027-59759960(+86)
Email:sales@eiaab.com