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Home  >  ELISA Kit  >  Rat NEFA ELISA Kit
NEFA (Shorten Name ),
Product Name:

Rat NEFA/ Nesfatin-1 ELISA Kit

Cat.#:

E8726r

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.156-10ng/mL
Sensitivity:
0.081ng/mL
Specificity:
Natural and recombinant rat Nesfatin-1
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Rat NEFA ELISA Kit
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Rat NEFA ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.1%

Recovery
Recovery was determined by spiking various levels of Nesfatin-1 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

80

80-85

Plasma

81

80-87

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Nesfatin-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

99-107%

104-115%

104-114%

95-104%

EDTA plasma(n=5)

110-119%

99-108%

82-82%

102-114%

heparin plasma(n=5)

103-113%

 

107-117%

101-111%

96-104%

 

General Annotation


Sub Unit:
N/A


Function:
N/A


Location:
N/A


This product has not yet been referenced specifically in any publications.
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