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NEFA (Shorten Name),
Product Name:

Human NEFA/ Nesfatin-1 ELISA Kit

 
Cat.#:

E8726h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.312-20ng/mL
Sensitivity:
0.12ng/mL
Specificity:
Natural and recombinant human Nesfatin-1
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Cancer
Product Overview:
E8726h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Nesfatin-1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Nesfatin-1. The ELISA analytical biochemical technique of the E8726h kit is based on Nesfatin-1 antibody-Nesfatin-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Nesfatin-1 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Nesfatin-1. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human NEFA ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.5%

Recovery
Recovery was determined by spiking various levels of Nesfatin-1 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

93

87-99

Plasma

95

89-101

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Nesfatin-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

108-118%

100-109%

119-129%

112-120%

EDTA plasma(n=5)

96-107%

112-112%

92-101%

95-104%

heparin plasma(n=5)

82-92%

 

108-117%

104-114%

103-113%

 

General Annotation


Sub Unit:
N/A


Function:
N/A


Location:
N/A


Sample Data
Sample Data
Sample Data
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