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MPO (Shorten Name),
Product Name:

Rat MPO/ Myeloperoxidase ELISA Kit

 
Cat.#:

E0601r

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.78-50ng/mL
Sensitivity:
0.44ng/mL
Specificity:
Natural and recombinant rat Myeloperoxidase
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Immunology
Product Overview:
E0601r is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Myeloperoxidase) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Myeloperoxidase. The ELISA analytical biochemical technique of the E0601r kit is based on Myeloperoxidase antibody-Myeloperoxidase antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Myeloperoxidase antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Myeloperoxidase. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Rat MPO ELISA Kit
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Product Datasheets
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.3%

Recovery
Recovery was determined by spiking various levels of Myeloperoxidase into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

97

91-103

Plasma

99

93-105

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Myeloperoxidase and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

95-106%

108-116%

88-97%

92-102%

EDTA plasma(n=5)

89-98%

108-117%

107-116%

90-102%

heparin plasma(n=5)

112-112%

 

116-116%

89-98%

107-117%

 

General Annotation


Sub Unit:
N/A


Function:
N/A


Location:
N/A



[1].
"Association between myeloperoxidase polymorphisms and its plasma levels with severity of coronary artery disease."

[2].
"No relationship between neutrophil granulocyte activation and the myeloperoxidase gene - 129 G>A and - 463 G>A promoter polymorphisms: implications for investigations of cardiovascular disease."

[3].
"Myeloperoxidase genotypes and enhanced efficacy of chemotherapy for early-stage breast cancer in SWOG-8897."

[4].
"A myeloperoxidase promoter polymorphism is independently associated with mortality in patients with impaired left ventricular function."

[5].
"A prospective study of genetic polymorphism in MPO, antioxidant status, and breast cancer risk."

[6].
"Myeloperoxidase and superoxide dismutase 2 polymorphisms comodulate the risk of hepatocellular carcinoma and death in alcoholic cirrhosis."

[7].
"Melatonin is a potent inhibitor for myeloperoxidase."

[8].
"Myeloperoxidase and superoxide dismutase polymorphisms are associated with an increased risk of developing pancreatic adenocarcinoma."

[9].
"Genetic polymorphisms in the oxidative stress pathway and susceptibility to non-Hodgkin lymphoma."

[10].
"Relationships between polymorphisms in NOS3 and MPO genes, cigarette smoking and risk of post-menopausal breast cancer."
Sample Data
Sample Data
Sample Data
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