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ACTH (Shorten Name ),
Product Name:

Rat ACTH/ Corticotropin ELISA Kit

Cat.#:

E0836r

Brand:
EIAab®
Regulatory Status:
Alternative:

Adrenocorticotropic hormone

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
15.6-1000pg/mL
Sensitivity:
7.8pg/mL
Specificity:
Natural and recombinant rat Corticotropin
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Neurosciences
Rat ACTH ELISA Kit
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Rat ACTH ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.8%

Recovery
Recovery was determined by spiking various levels of Corticotropin into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

101

95-107

Plasma

103

97-109

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Corticotropin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

108-118%

102-112%

100-100%

85-97%

EDTA plasma(n=5)

94-103%

110-122%

105-117%

80-93%

heparin plasma(n=5)

106-116%

 

106-114%

85-98%

106-116%

 

General Annotation


Sub Unit:
N/A


Function:
Its principal effects are increased production and release of corticosteroids and, as its name suggests, cortisol from the adrenal cortex.


Location:
Adrenocorticotropic hormone (ACTH), also known as 'corticotropin', 'Adrenocorticotrophic hormone', is a polypeptide tropic hormone produced and secreted by the anterior pituitary gland. It is an important component of the hypothalamic-pituitary-adrenal axis and is often produced in response to biological stress (along with corticotropin-releasing hormone from the hypothalamus).


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