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ACTH (Shorten Name),

Product Name:

Rat ACTH/ Corticotropin ELISA Kit

Cat.#:

E0836r

Brand:

EIAab^®

Regulatory Status:

RUO

Alternative:

Adrenocorticotropic hormone

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

15.6-1000pg/mL

Sensitivity:

7.8pg/mL

Specificity:

Natural and recombinant rat Corticotropin

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Neurosciences

Product Overview:

E0836r is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Corticotropin) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Corticotropin. The ELISA analytical biochemical technique of the E0836r kit is based on Corticotropin antibody-Corticotropin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Corticotropin antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Corticotropin. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

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Rat ACTH ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations ( 1 )
Sequence / 3D
Feedback Wall (0)

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.8%

Recovery

Recovery was determined by spiking various levels of Corticotropin into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	101	95-107
Plasma	103	97-109

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Corticotropin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	108-118%	102-112%	100-100%	85-97%
EDTA plasma(n=5)	94-103%	110-122%	105-117%	80-93%
heparin plasma(n=5)	106-116%	106-114%	85-98%	106-116%

General Annotation

Sub Unit:

N/A

Function:

Its principal effects are increased production and release of corticosteroids and, as its name suggests, cortisol from the adrenal cortex.

Location:

Adrenocorticotropic hormone (ACTH), also known as 'corticotropin', 'Adrenocorticotrophic hormone', is a polypeptide tropic hormone produced and secreted by the anterior pituitary gland. It is an important component of the hypothalamic-pituitary-adrenal axis and is often produced in response to biological stress (along with corticotropin-releasing hormone from the hypothalamus).

Database links

Uniprot:

P01194

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