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C-Peptide (Shorten Name),

Product Name:

Rat C-Peptide ELISA Kit

Cat.#:

E0447r

Brand:

EIAab^®

Regulatory Status:

RUO

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

78-5000pg/mL

Sensitivity:

39pg/mL

Specificity:

Natural and recombinant rat C-Peptide

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Metabolism

Product Overview:

E0447r is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (C-Peptide) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing C-Peptide. The ELISA analytical biochemical technique of the E0447r kit is based on C-Peptide antibody-C-Peptide antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C-Peptide antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, C-Peptide. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

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Rat C-Peptide ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations ( 1 )
Sequence / 3D
Feedback Wall (0)

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.2%

Recovery

Recovery was determined by spiking various levels of C-Peptide into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	95	89-101
Plasma	97	91-103

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C-Peptide and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	82-90%	107-117%	90-98%	100-112%
EDTA plasma(n=5)	96-106%	110-119%	107-116%	112-120%
heparin plasma(n=5)	81-91%	95-104%	106-106%	94-106%

General Annotation

Sub Unit:

N/A

Function:

C-peptide is a protein that is produced in the body along with insulin. First preproinsulin is secreted with an A-chain, C-peptide, a B-chain, and a signal sequence. The signal sequence is cut off, leaving proinsulin. Then the C-peptide is cut off, leaving the A-chain and B-chain to form insulin.

Location:

Cellular effects of C-peptide: C-peptide has been shown to bind to the surface of a number of cell types such as neuronal, endothelial, fibroblast and renal tubular, at nanomolar concentrations to a receptor that is likely G-protein-coupled. The signal activates Ca2+-dependent intracellular signaling pathways such as MAPK, PLCγ, and PKC, leading to upregulation of a range of transcription factors as well as eNOS and Na+K+ATPase activities.

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