C-Peptide (Shorten Name ),
Product Name:
Rat C-Peptide ELISA Kit
Cat.#:
E0447r
Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
78-5000pg/mL
Sensitivity:
39pg/mL
Specificity:
Natural and recombinant rat C-Peptide
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:

Research Area:
Metabolism
- Data
- Citations ( 1 )
- Sequence / 3D
- Feedback Wall (0)
Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤5.3%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤8.2%
Recovery
Recovery was determined by spiking various levels of C-Peptide into serum and plasma.
Sample Type |
Average(%) |
Recovery Range(%) |
Serum |
95 |
89-101 |
Plasma |
97 |
91-103 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C-Peptide and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
serum(n=5) |
82-90% |
107-117% |
90-98% |
100-112% |
EDTA plasma(n=5) |
96-106% |
110-119% |
107-116% |
112-120% |
heparin plasma(n=5) |
81-91%
|
95-104% |
106-106% |
94-106% |
General Annotation
Sub Unit:
N/A
Function:
C-peptide is a protein that is produced in the body along with insulin. First preproinsulin is secreted with an A-chain, C-peptide, a B-chain, and a signal sequence. The signal sequence is cut off, leaving proinsulin. Then the C-peptide is cut off, leaving the A-chain and B-chain to form insulin.
Location:
Cellular effects of C-peptide: C-peptide has been shown to bind to the surface of a number of cell types such as neuronal, endothelial, fibroblast and renal tubular, at nanomolar concentrations to a receptor that is likely G-protein-coupled. The signal activates Ca2+-dependent intracellular signaling pathways such as MAPK, PLCγ, and PKC, leading to upregulation of a range of transcription factors as well as eNOS and Na+K+ATPase activities.
[1].
Do-Young Park, Young-Tae Ahn, Chul-Sung Huh, Robin A McGregor, and Myung-Sook Choi
From:
World J Gastroenterol
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