027-59234612(+86)
EIAab
Home  >  ELISA Kit  >  Mouse C-Peptide ELISA Kit
C-Peptide (Shorten Name),
Product Name:

Mouse C-Peptide ELISA Kit

 
Cat.#:

E0447m

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.156-10ng/ml
Sensitivity:
0.083ng/mL
Specificity:
Natural and recombinant mouse C-Peptide
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Metabolism
Product Overview:
E0447m is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (C-Peptide) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing C-Peptide. The ELISA analytical biochemical technique of the E0447m kit is based on C-Peptide antibody-C-Peptide antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C-Peptide antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, C-Peptide. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
More
Mouse C-Peptide ELISA Kit
Package & Price: Sign in.
Attention Attention
×
Mouse C-Peptide ELISA Kit
Email *
Message *
Please Sign in.
Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.2%

Recovery
Recovery was determined by spiking various levels of C-Peptide into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

106

100-112

Plasma

108

102-114

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C-Peptide and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

98-107%

82-92%

82-91%

96-106%

EDTA plasma(n=5)

101-111%

108-117%

98-109%

84-95%

heparin plasma(n=5)

114-124%

 

100-110%

88-98%

93-104%

 

General Annotation


Sub Unit:
N/A


Function:
C-peptide is a protein that is produced in the body along with insulin. First preproinsulin is secreted with an A-chain, C-peptide, a B-chain, and a signal sequence. The signal sequence is cut off, leaving proinsulin. Then the C-peptide is cut off, leaving the A-chain and B-chain to form insulin.


Location:
Cellular effects of C-peptide: C-peptide has been shown to bind to the surface of a number of cell types such as neuronal, endothelial, fibroblast and renal tubular, at nanomolar concentrations to a receptor that is likely G-protein-coupled. The signal activates Ca2+-dependent intracellular signaling pathways such as MAPK, PLCγ, and PKC, leading to upregulation of a range of transcription factors as well as eNOS and Na+K+ATPase activities.


This product has not yet been referenced specifically in any publications.
Publishing research using this product ?
Please let us know and we will provide a credit for your next order.
Sample Data
Sample Data
Sample Data
My Cart close
Attention close
History close
Clear All
Comparison close
User
My Cart
Attention
History
Comparison
Top
Message
close_message
Product Name: *
Email: *
Message: *
Inform
new Message
Package Quantity Price ($) Subtotal 1 ($)
Subtotal 2: $
triangle
Package Quantity Price ($)
Do you want to be our agent and get a lower discount?
Please contact us:
Tel:027-59234612(+86)
Fax:027-59759960(+86)
Email:sales@eiaab.com