C-Peptide (Shorten Name),
Product Name:
Mouse C-Peptide ELISA Kit
Cat.#:
E0447m
Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.156-10ng/mL
Sensitivity:
0.083ng/mL
Specificity:
Natural and recombinant mouse C-Peptide
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Metabolism
Product Overview:
E0447m is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay)
Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (C-Peptide) ELISA Kit target analytes in biological samples. The concentration gradients of
the kit standards or positive controls render a theoretical kit detection range in biological research samples containing C-Peptide. The ELISA analytical biochemical
technique of the E0447m kit is based on C-Peptide antibody-C-Peptide antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C-Peptide
antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, C-Peptide. Appropriate sample types may include undiluted human body fluids
and/or tissue homogenates,
secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.3%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤8.2%
Recovery
Recovery was determined by spiking various levels of C-Peptide into serum and plasma.
Sample Type | Average(%) | Recovery Range(%) |
Serum | 106 | 100-112 |
Plasma | 108 | 102-114 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C-Peptide and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1：2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 98-107% | 82-92% | 82-91% | 96-106% |
EDTA plasma(n=5) | 101-111% | 108-117% | 98-109% | 84-95% |
heparin plasma(n=5) | 114-124%
| 100-110% | 88-98% | 93-104% |
General Annotation
Sub Unit:
N/A
Function:
C-peptide is a protein that is produced in the body along with insulin. First preproinsulin is secreted with an A-chain, C-peptide, a B-chain, and a signal sequence. The signal sequence is cut off, leaving proinsulin. Then the C-peptide is cut off, leaving the A-chain and B-chain to form insulin.
Location:
Cellular effects of C-peptide: C-peptide has been shown to bind to the surface of a number of cell types such as neuronal, endothelial, fibroblast and renal tubular, at nanomolar concentrations to a receptor that is likely G-protein-coupled. The signal activates Ca2+-dependent intracellular signaling pathways such as MAPK, PLCγ, and PKC, leading to upregulation of a range of transcription factors as well as eNOS and Na+K+ATPase activities.
Vaild Sequence:
This product has not yet been referenced specifically in any publications.
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Publishing research using this product ?
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