C-Peptide (Shorten Name ),
Product Name:
Dog C-Peptide ELISA Kit
Cat.#:
E0447d
Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.156-10ng/mL
Sensitivity:
0.069ng/mL
Specificity:
Natural and recombinant dog C-Peptide
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:

Research Area:
Metabolism
- Data
- Citations
- Sequence / 3D
- Feedback Wall (0)
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C-Peptide and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
serum(n=5) |
99-109% |
93-105% |
85-97% |
90-100% |
EDTA plasma(n=5) |
87-97% |
105-115% |
98-108% |
96-106% |
heparin plasma(n=5) |
101-110%
|
99-109% |
106-116% |
101-110% |
General Annotation
Sub Unit:
N/A
Function:
C-peptide is a protein that is produced in the body along with insulin. First preproinsulin is secreted with an A-chain, C-peptide, a B-chain, and a signal sequence. The signal sequence is cut off, leaving proinsulin. Then the C-peptide is cut off, leaving the A-chain and B-chain to form insulin.
Location:
Cellular effects of C-peptide: C-peptide has been shown to bind to the surface of a number of cell types such as neuronal, endothelial, fibroblast and renal tubular, at nanomolar concentrations to a receptor that is likely G-protein-coupled. The signal activates Ca2+-dependent intracellular signaling pathways such as MAPK, PLCγ, and PKC, leading to upregulation of a range of transcription factors as well as eNOS and Na+K+ATPase activities.
This product has not yet been referenced specifically in any publications.
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