Mouse PINP/ N-terminal propeptide of Collagen alpha-1(I) chain ELISA Kit

Cat.#:

E0957m

Brand:

EIAab^®

Regulatory Status:

RUO

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

78-5000pg/mL

Sensitivity:

39.7pg/mL

Specificity:

Natural and recombinant mouse N-terminal propeptide of Collagen alpha-1(I) chain

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

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Mouse PINP ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations
Sequence / 3D
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Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.9%

Recovery

Recovery was determined by spiking various levels of N-terminal propeptide of Collagen alpha-1(I) chain into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	86	80-92
Plasma	88	82-94

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of N-terminal propeptide of Collagen alpha-1(I) chain and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	104-114%	93-102%	110-120%	107-116%
EDTA plasma(n=5)	106-116%	101-111%	103-114%	107-116%
heparin plasma(n=5)	98-108%	82-91%	95-104%	104-113%