PINP (Shorten Name),
Product Name:
Mouse PINP/ N-terminal propeptide of Collagen alpha-1(I) chain ELISA Kit
Cat.#:
E0957m
Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
78-5000pg/mL
Sensitivity:
39.7pg/mL
Specificity:
Natural and recombinant mouse N-terminal propeptide of Collagen alpha-1(I) chain
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0957m is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay)
Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (N-terminal propeptide of Collagen alpha-1(I) chain) ELISA Kit target analytes in biological samples. The concentration gradients of
the kit standards or positive controls render a theoretical kit detection range in biological research samples containing N-terminal propeptide of Collagen alpha-1(I) chain. The ELISA analytical biochemical
technique of the E0957m kit is based on N-terminal propeptide of Collagen alpha-1(I) chain antibody-N-terminal propeptide of Collagen alpha-1(I) chain antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect N-terminal propeptide of Collagen alpha-1(I) chain
antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, N-terminal propeptide of Collagen alpha-1(I) chain. Appropriate sample types may include undiluted human body fluids
and/or tissue homogenates,
secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤7.5%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤9.9%
Recovery
Recovery was determined by spiking various levels of N-terminal propeptide of Collagen alpha-1(I) chain into serum and plasma.
|
Sample Type |
Average(%) |
Recovery Range(%) |
|
Serum |
86 |
80-92 |
|
Plasma |
88 |
82-94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of N-terminal propeptide of Collagen alpha-1(I) chain and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1：2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
104-114% |
93-102% |
110-120% |
107-116% |
|
EDTA plasma(n=5) |
106-116% |
101-111% |
103-114% |
107-116% |
|
heparin plasma(n=5) |
98-108%
|
82-91% |
95-104% |
104-113% |
General Annotation
Sub Unit:
N/A
Function:
N/A
Location:
N/A
Vaild Sequence:
This product has not yet been referenced specifically in any publications.
Publishing research using this product ?
Please let us know and we will provide a credit for your next order.
Publishing research using this product ?
Please let us know and we will provide a credit for your next order.
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Fax:
Email:sales@eiaab.com