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IgM (Shorten Name),
Product Name:

Dog IgM/ Immunoglobulin M ELISA Kit

 
Cat.#:

E0543d

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
3.12-200ng/mL
Sensitivity:
0.16 ng/mL
Specificity:
Natural and recombinant dog Immunoglobulin M
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Immunology
Product Overview:
E0543d is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Immunoglobulin M) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Immunoglobulin M. The ELISA analytical biochemical technique of the E0543d kit is based on Immunoglobulin M antibody-Immunoglobulin M antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Immunoglobulin M antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Immunoglobulin M. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Dog IgM ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Immunoglobulin M and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

104-113%

111-120%

99-109%

94-103%

EDTA plasma(n=5)

87-97%

89-98%

102-114%

100-109%

heparin plasma(n=5)

99-109%

 

99-110%

84-97%

108-116%

 

General Annotation


Sub Unit:
N/A


Function:
Due to its polymeric nature, IgM possesses high avidity, and is particularly effective at complement activation. By itself, IgM is an ineffective opsonin; however it contributes greatly to opsonization by activating complement and causing C3b to bind to the antigen.


Location:
Due to its polymeric nature, IgM possesses high avidity, and is particularly effective at complement activation. By itself, IgM is an ineffective opsonin; however it contributes greatly to opsonization by activating complement and causing C3b to bind to the antigen.


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