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ACTH (Shorten Name),
Product Name:

Salmon ACTH/ Corticotropin ELISA Kit

 
Cat.#:

E0836s

Brand:
EIAab®
Regulatory Status:
Alternative:

Adrenocorticotropic hormone

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
15.6-1000pg/mL
Sensitivity:
5.5pg/mL
Specificity:
Natural and recombinant salmon Corticotropin
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Neurosciences
Product Overview:
E0836s is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Corticotropin) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Corticotropin. The ELISA analytical biochemical technique of the E0836s kit is based on Corticotropin antibody-Corticotropin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Corticotropin antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Corticotropin. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Salmon ACTH ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Corticotropin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

88-97%

87-97%

110-120%

90-100%

EDTA plasma(n=5)

82-91%

118-127%

103-111%

90-99%

heparin plasma(n=5)

108-120%

 

90-98%

90-100%

96-105%

 

General Annotation


Sub Unit:
N/A


Function:
Its principal effects are increased production and release of corticosteroids and, as its name suggests, cortisol from the adrenal cortex.


Location:
Adrenocorticotropic hormone (ACTH), also known as 'corticotropin', 'Adrenocorticotrophic hormone', is a polypeptide tropic hormone produced and secreted by the anterior pituitary gland. It is an important component of the hypothalamic-pituitary-adrenal axis and is often produced in response to biological stress (along with corticotropin-releasing hormone from the hypothalamus).


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