027-59234612(+86)
EIAab
Home  >  ELISA Kit  >  Mouse IgE ELISA Kit
IgE (Shorten Name ),
Product Name:

Mouse IgE/ Immunoglobulin E ELISA Kit

Cat.#:

E0545m

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
1.56-100ng/mL
Sensitivity:
0.65ng/mL
Specificity:
Natural and recombinant mouse Immunoglobulin E
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Immunology
Mouse IgE ELISA Kit
Size & Price: Sign In.
×
Mouse IgE ELISA Kit
Email *
Message *
Please Sign In.
Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.5%

Recovery
Recovery was determined by spiking various levels of Immunoglobulin E into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

80

80-83

Plasma

80

80-85

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Immunoglobulin E and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

98-108%

88-100%

114-123%

104-114%

EDTA plasma(n=5)

104-114%

117-117%

93-103%

104-117%

heparin plasma(n=5)

97-108%

 

91-102%

86-95%

109-119%

 

General Annotation


Sub Unit:
N/A


Function:
Although IgE is typically the least abundant isotype - blood serum IgE levels in a normal ("non-atopic") individual are only 0.05% of the Ig concentration, compared to 10?mg/ml for the IgGs (the isotypes responsible for most of the classical adaptive immune response) - it is capable of triggering the most powerful inflammation reactions.


Location:
Immunoglobulin E (IgE) is a class of antibody (or immunoglobulin "isotype") that has been found only in mammals. IgE is a monomeric antibody with 4 Ig-like domains (Cε1-Cε4).


Close
Sample Data
Close
Sample Data
Close
Sample Data
My Cart close
Attention close
History close
Clear All
Comparison close
User
My Cart
Attention
History
Comparison
Top
Message
close_message
Product Name *
Email *
Message: *
Inform
new Message
Size Quantity Price ($) Subtotal 1 ($)
Subtotal 2: $
triangle
Size Quantity Price ($)
Do you want to be our agent and get a lower discount?
Please contact us:
Tel:027-59234612(+86)
Fax:027-59759960(+86)
Email:sales@eiaab.com