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VB1 (Shorten Name),
Product Name:

General VB1/ Vitamin B1 ELISA Kit

 
Cat.#:

E2109Ge

Brand:
EIAab®
Regulatory Status:
Alternative:

Thiamine,thiamin

Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
1.56-100ng/mL
Sensitivity:
1ng/mL
Specificity:
Natural and recombinant general Vitamin B1
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E2109Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Vitamin B1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Vitamin B1. The ELISA analytical biochemical technique of the E2109Ge kit is based on Vitamin B1 antibody-Vitamin B1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Vitamin B1 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Vitamin B1. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General VB1 ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: 3.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: 6.5%

Recovery
Recovery was determined by spiking various levels of Vitamin B1 into serum and plasma.

Recovery was determined by spiking various levels of VB1 into serum and plasma .

Sample Type

Average(%)

Recovery Range(%)

Serum

99

96-103

Plasma

98

97-106

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Vitamin B1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

93-103%

100-100%

92-102%

96-106%

EDTA plasma(n=5)

107-117%

111-120%

88-101%

113-123%

heparin plasma(n=5)

97-106%

 

107-117%

96-106%

96-108%

 

General Annotation


Sub Unit:
N/A


Function:
vitamin B1, named as the "thio-vitamine" ("sulfur-containing vitamin") is a water-soluble vitamin of the B complex. First named aneurin for the detrimental neurological effects if not present in the diet, it was eventually assigned the generic descriptor name vitamin B1. Its phosphate derivatives are involved in many cellular processes. The best-characterized form is thiamine pyrophosphate (TPP), a coenzyme in the catabolism of sugars and amino acids. Thiamine is used in the biosynthesis of the neurotransmitter acetylcholine and gamma-aminobutyric acid (GABA). In yeast, TPP is also required in the first step of alcoholic fermentation. All living organisms use thiamine in their biochemistry, but it is only synthesized in bacteria, fungi, and plants. Animals must obtain it from their diet, and, thus, for them, it is an essential nutrient. Insufficient intake in birds produces a characteristic polyneuritis. In mammals, deficiency results in Korsakoff's syndrome, optic neuropathy, and a disease called beriberi that affects the peripheral nervous system (polyneuritis) and/or the cardiovascular system. Thiamine deficiency has a potentially fatal outcome if it remains untreated. In less severe cases, nonspecific signs include malaise, weight loss, irritability and confusion. There is still much research devoted to elucidating the exact mechanisms by which thiamine deficiency leads to the specific symptoms observed. New thiamine phosphate derivatives have recently been discovered,emphasizing the complexity of thiamine metabolism.


Location:
N/A


This product has not yet been referenced specifically in any publications.
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