027-59234612(+86)
EIAab
Home  >  ELISA Kit  >  General VB1 ELISA Kit
VB1 (Shorten Name ),
Product Name:

General VB1/ Vitamin B1 ELISA Kit

Cat.#:

E2109Ge

Brand:
EIAab®
Regulatory Status:
Alternative:

Thiamine, thiamin

Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
1.56-100ng/mL
Sensitivity:
1ng/mL
Specificity:
Natural and recombinant general Vitamin B1
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
General VB1 ELISA Kit
Size & Price: Sign In.
×
General VB1 ELISA Kit
Email *
Message *
Please Sign In.
Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: 3.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: 6.5%

Recovery
Recovery was determined by spiking various levels of Vitamin B1 into serum and plasma.

Recovery was determined by spiking various levels of VB1 into serum and plasma .

Sample Type

Average(%)

Recovery Range(%)

Serum

99

96-103

Plasma

98

97-106

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Vitamin B1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

93-103%

100-100%

92-102%

96-106%

EDTA plasma(n=5)

107-117%

111-120%

88-101%

113-123%

heparin plasma(n=5)

97-106%

 

107-117%

96-106%

96-108%

 

General Annotation


Sub Unit:
N/A


Function:
vitamin B1, named as the "thio-vitamine" ("sulfur-containing vitamin") is a water-soluble vitamin of the B complex. First named aneurin for the detrimental neurological effects if not present in the diet, it was eventually assigned the generic descriptor name vitamin B1. Its phosphate derivatives are involved in many cellular processes. The best-characterized form is thiamine pyrophosphate (TPP), a coenzyme in the catabolism of sugars and amino acids. Thiamine is used in the biosynthesis of the neurotransmitter acetylcholine and gamma-aminobutyric acid (GABA). In yeast, TPP is also required in the first step of alcoholic fermentation. All living organisms use thiamine in their biochemistry, but it is only synthesized in bacteria, fungi, and plants. Animals must obtain it from their diet, and, thus, for them, it is an essential nutrient. Insufficient intake in birds produces a characteristic polyneuritis. In mammals, deficiency results in Korsakoff's syndrome, optic neuropathy, and a disease called beriberi that affects the peripheral nervous system (polyneuritis) and/or the cardiovascular system. Thiamine deficiency has a potentially fatal outcome if it remains untreated. In less severe cases, nonspecific signs include malaise, weight loss, irritability and confusion. There is still much research devoted to elucidating the exact mechanisms by which thiamine deficiency leads to the specific symptoms observed. New thiamine phosphate derivatives have recently been discovered,emphasizing the complexity of thiamine metabolism.


Location:
N/A


This product has not yet been referenced specifically in any publications.
Close
Sample Data
Close
Sample Data
Close
Sample Data
My Cart close
Attention close
History close
Clear All
Comparison close
User
My Cart
Attention
History
Comparison
Top
Message
close_message
Product Name *
Email *
Message: *
Inform
new Message
Size Quantity Price ($) Subtotal 1 ($)
Subtotal 2: $
triangle
Size Quantity Price ($)
Do you want to be our agent and get a lower discount?
Please contact us:
Tel:027-59234612(+86)
Fax:027-59759960(+86)
Email:sales@eiaab.com