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首页  >  酶联免疫试剂盒  >  General VB1 ELISA Kit
VB1 (短名 ),
产品名称:

General VB1/ Vitamin B1 ELISA Kit
维生素B1

货号:

E2109Ge

商标:
EIAab®
监管等级:
别名:

Thiamine, thiamin

检测方法:
ELISA
实验类型:
Competitive
检测范围:
1.56-100ng/mL
灵敏度:
0.89ng/mL
特异性:
Natural and recombinant general Vitamin B1
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
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General VB1 ELISA Kit
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产品说明书
数据表: 下载说明书
说明书: 下载说明书
MSDS: MSDS


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: 3.7%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: 6.5%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Recovery was determined by spiking various levels of VB1 into serum and plasma .

Sample Type

Average(%)

Recovery Range(%)

Serum

99

96-103

Plasma

98

97-106

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

93-103%

100-100%

92-102%

96-106%

EDTA plasma(n=5)

107-117%

111-120%

88-101%

113-123%

heparin plasma(n=5)

97-106%

 

107-117%

96-106%

96-108%

 

通用注释


亚单元:
N/A


功能:
vitamin B1, named as the "thio-vitamine" ("sulfur-containing vitamin") is a water-soluble vitamin of the B complex. First named aneurin for the detrimental neurological effects if not present in the diet, it was eventually assigned the generic descriptor name vitamin B1. Its phosphate derivatives are involved in many cellular processes. The best-characterized form is thiamine pyrophosphate (TPP), a coenzyme in the catabolism of sugars and amino acids. Thiamine is used in the biosynthesis of the neurotransmitter acetylcholine and gamma-aminobutyric acid (GABA). In yeast, TPP is also required in the first step of alcoholic fermentation. All living organisms use thiamine in their biochemistry, but it is only synthesized in bacteria, fungi, and plants. Animals must obtain it from their diet, and, thus, for them, it is an essential nutrient. Insufficient intake in birds produces a characteristic polyneuritis. In mammals, deficiency results in Korsakoff's syndrome, optic neuropathy, and a disease called beriberi that affects the peripheral nervous system (polyneuritis) and/or the cardiovascular system. Thiamine deficiency has a potentially fatal outcome if it remains untreated. In less severe cases, nonspecific signs include malaise, weight loss, irritability and confusion. There is still much research devoted to elucidating the exact mechanisms by which thiamine deficiency leads to the specific symptoms observed. New thiamine phosphate derivatives have recently been discovered,emphasizing the complexity of thiamine metabolism.


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