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TAP (Shorten Name),
Product Name:

Human TAP/ Trypsinogen Activation Peptide,TAP ELISA Kit

 
Cat.#:

E0634h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.78-50ng/mL
Sensitivity:
0.444ng/mL
Specificity:
Natural and recombinant human Trypsinogen Activation Peptide,TAP
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0634h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Trypsinogen Activation Peptide,TAP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Trypsinogen Activation Peptide,TAP. The ELISA analytical biochemical technique of the E0634h kit is based on Trypsinogen Activation Peptide,TAP antibody-Trypsinogen Activation Peptide,TAP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Trypsinogen Activation Peptide,TAP antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Trypsinogen Activation Peptide,TAP. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human TAP ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤5.5%

Recovery
Recovery was determined by spiking various levels of Trypsinogen Activation Peptide,TAP into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

86

80-92

Plasma

88

82-94

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Trypsinogen Activation Peptide,TAP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

85-95%

105-115%

105-117%

115-125%

EDTA plasma(n=5)

80-90%

100-110%

103-103%

89-99%

heparin plasma(n=5)

83-95%

 

95-107%

118-118%

96-105%

 

General Annotation


Sub Unit:
N/A


Function:
Trypsinogen-activation peptide (TAP) is a small peptide that is released when trypsinogen is activated to trypsin. Under physiologic conditions, activation of trypsinogen occurs in the intestinal lumen and is catalyzed by enteropeptidase (formerly known as enterokinase). In the intestinal lumen, TAP is quickly degraded by peptidases of the brush border membrane. In pancreatitis, trypsinogen is prematurely activated within pancreatic acinar cells, and TAP is released into the peripheral circulation. Although TAP is quickly excreted through the kidneys, with a circulating half-life of less than 8 min, significant increases in plasma and urine TAP concentrations have been reported in canine and human patients with acute pancreatitis. An assay of TAP has been shown to be useful for the diagnosis and staging of pancreatitis in humans and dogs. Also, measurement of TAP in plasma and urine has been evaluated in experimental rodent and feline models of pancreatitis and has been shown to be useful in assessing pancreatic inflammation in those species.


Location:
N/A


This product has not yet been referenced specifically in any publications.
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