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VK1 (Shorten Name),
Product Name:

General VK1/ Phylloquinone ELISA Kit

 
Cat.#:

E0926Ge

Brand:
EIAab®
Regulatory Status:
Alternative:

Vitamin K1

Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.156-10ng/mL
Sensitivity:
0.05ng/mL
Specificity:
Natural and recombinant general Phylloquinone
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0926Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Phylloquinone) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Phylloquinone. The ELISA analytical biochemical technique of the E0926Ge kit is based on Phylloquinone antibody-Phylloquinone antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Phylloquinone antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Phylloquinone. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General VK1 ELISA Kit
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Product Datasheets
Instruction:
MSDS:


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Phylloquinone and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

80-92%

96-107%

104-114%

101-113%

EDTA plasma(n=5)

102-110%

107-117%

89-98%

92-102%

heparin plasma(n=5)

114-114%

 

87-99%

103-112%

102-112%

 

General Annotation


Sub Unit:
N/A


Function:
It is often called vitamin K1, phytomenadione or phytonadione. Sometimes a distinction is made with phylloquinone considered natural and phytonadione considered synthetic. Phylloquinone is an electron acceptor during photosynthesis, forming part of the electron transport chain of Photosystem I. Its best-known function in animals is as a cofactor in the formation of coagulation factors II (prothrombin), VII, IX, and X by the liver. It is also required for the formation of anticoagulant factors protein C and S. It is commonly used to treat warfarin toxicity, and as an antidote for coumatetralyl. Vitamin K is also required for bone protein formation.


Location:
N/A


This product has not yet been referenced specifically in any publications.
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