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LDL (Shorten Name),
Product Name:

Rat LDL/ Low-density lipoprotein ELISA Kit

 
Cat.#:

E1107r

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
6.25-400ng/mL
Sensitivity:
3.165ng/mL
Specificity:
Natural and recombinant rat Low-density lipoprotein
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E1107r is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Low-density lipoprotein) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Low-density lipoprotein. The ELISA analytical biochemical technique of the E1107r kit is based on Low-density lipoprotein antibody-Low-density lipoprotein antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Low-density lipoprotein antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Low-density lipoprotein. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Rat LDL ELISA Kit
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Product Datasheets
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.7%

Recovery
Recovery was determined by spiking various levels of Low-density lipoprotein into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

106

100-112

Plasma

108

102-114

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Low-density lipoprotein and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

100-109%

96-106%

107-117%

109-119%

EDTA plasma(n=5)

104-114%

104-113%

84-95%

105-114%

heparin plasma(n=5)

92-102%

 

85-93%

101-111%

100-110%

 

General Annotation


Sub Unit:
N/A


Function:
Low-density lipoprotein (LDL) is one of the five major groups of lipoproteins, which in order of size, largest to smallest, are chylomicrons, VLDL, IDL, LDL, and HDL, that enable transport of multiple different fat molecules, including cholesterol, within the water around cells and within the water-based bloodstream.


Location:
N/A


This product has not yet been referenced specifically in any publications.
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Please let us know and we will provide a credit for your next order.
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