Mouse GADAb/ Glutamate decarboxylase autoantibody ELISA Kit

Cat.#:

E0859m

Brand:

EIAab^®

Regulatory Status:

RUO

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

1.56-100U/mL

Sensitivity:

0.782U/mL

Specificity:

Natural and recombinant mouse Glutamate decarboxylase autoantibody

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Product Overview:

E0859m is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Glutamate decarboxylase autoantibody) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Glutamate decarboxylase autoantibody. The ELISA analytical biochemical technique of the E0859m kit is based on Glutamate decarboxylase autoantibody antibody-Glutamate decarboxylase autoantibody antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Glutamate decarboxylase autoantibody antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Glutamate decarboxylase autoantibody. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

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Mouse GADAb ELISA Kit

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Product Datasheets

Instruction:

MSDS:

Data
Citations ( 1 )
Sequence / 3D
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Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤8.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.6%

Recovery

Recovery was determined by spiking various levels of Glutamate decarboxylase autoantibody into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	111	105-117
Plasma	113	107-119

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glutamate decarboxylase autoantibody and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	101-111%	81-90%	106-118%	101-112%
EDTA plasma(n=5)	84-93%	83-93%	104-114%	112-124%
heparin plasma(n=5)	101-109%	95-105%	90-99%	92-102%

General Annotation

Sub Unit:

N/A

Function:

Glutamate decarboxylase or glutamic acid decarboxylase (GAD) is an enzyme that catalyzes the decarboxylation of glutamate to GABA and CO2. GAD uses PLP as a cofactor. Intracerebellar administration of GAD autoantibodies to animals increase the excitability of motoneurons and impairs the production of nitric oxide (NO), a molecule involved in learning. Epitope recognition contributes to cerebellar involvement.

Location:

N/A

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