Mouse GADAb/ Glutamate decarboxylase autoantibody ELISA Kit

Cat.#:

E0859m

Brand:

EIAab^®

Regulatory Status:

RUO

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

1.56-100U/mL

Sensitivity:

0.782U/mL

Specificity:

Natural and recombinant mouse Glutamate decarboxylase autoantibody

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

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Mouse GADAb ELISA Kit

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Product Datasheets

Instruction:

MSDS:

Data
Citations ( 1 )
Sequence / 3D
Feedback Wall (0)

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤8.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.6%

Recovery

Recovery was determined by spiking various levels of Glutamate decarboxylase autoantibody into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	111	105-117
Plasma	113	107-119

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glutamate decarboxylase autoantibody and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	101-111%	81-90%	106-118%	101-112%
EDTA plasma(n=5)	84-93%	83-93%	104-114%	112-124%
heparin plasma(n=5)	101-109%	95-105%	90-99%	92-102%

General Annotation

Sub Unit:

N/A

Function:

Glutamate decarboxylase or glutamic acid decarboxylase (GAD) is an enzyme that catalyzes the decarboxylation of glutamate to GABA and CO2. GAD uses PLP as a cofactor. Intracerebellar administration of GAD autoantibodies to animals increase the excitability of motoneurons and impairs the production of nitric oxide (NO), a molecule involved in learning. Epitope recognition contributes to cerebellar involvement.

Location:

N/A

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