General VD3/ Vitamin D3 ELISA Kit

Cat.#:

E0920Ge

Brand:

EIAab^®

Regulatory Status:

RUO

Alternative:

Cholecalciferol

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

1.56-100ng/mL

Sensitivity:

0.4ng/mL

Specificity:

Natural and recombinant general Vitamin D3

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Product Overview:

E0920Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Vitamin D3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Vitamin D3. The ELISA analytical biochemical technique of the E0920Ge kit is based on Vitamin D3 antibody-Vitamin D3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Vitamin D3 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Vitamin D3. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

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General VD3 ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations ( 2 )
Sequence / 3D
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Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Vitamin D3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	99-109%	90-99%	93-103%	108-119%
EDTA plasma(n=5)	106-114%	86-97%	109-119%	92-102%
heparin plasma(n=5)	106-116%	102-111%	99-108%	105-117%

General Annotation

Sub Unit:

N/A

Function:

Cholecalciferol is a form of vitamin D, also called vitamin D3. Vitamin D3 has several forms: Cholecalciferol, (sometimes called calciol) is an inactive, unhydroxylated form of vitamin D3; Calcifediol (also called calcidiol, hydroxycholecalciferol, 25-hydroxyvitamin D3, etc. and abbreviated 25(OH)D) is one of the forms measured in the blood to assess vitamin D status; Calcitriol (also called 1,25-dihydroxyvitamin D3) is the active form of D3.

Location:

N/A

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