027-59234612(+86)
EIAab
Home  >  ELISA Kit  >  Mouse ACTH ELISA Kit
ACTH (Shorten Name),
Product Name:

Mouse ACTH/ Corticotropin ELISA Kit

 
Cat.#:

E0836m

Brand:
EIAab®
Regulatory Status:
Alternative:

Adrenocorticotropic hormone

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
7.8-500pg/mL
Sensitivity:
3.93pg/mL
Specificity:
Natural and recombinant mouse Corticotropin
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Neurosciences
Product Overview:
E0836m is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Corticotropin) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Corticotropin. The ELISA analytical biochemical technique of the E0836m kit is based on Corticotropin antibody-Corticotropin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Corticotropin antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Corticotropin. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
More
Mouse ACTH ELISA Kit
Package & Price: Sign in.
Attention Attention
×
Mouse ACTH ELISA Kit
Email *
Message *
Please Sign in.
Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.2%

Recovery
Recovery was determined by spiking various levels of Corticotropin into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

99

93-105

Plasma

101

95-107

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Corticotropin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

96-106%

82-94%

82-92%

89-99%

EDTA plasma(n=5)

86-95%

108-118%

102-115%

87-99%

heparin plasma(n=5)

80-92%

 

115-125%

101-111%

109-119%

 

General Annotation


Sub Unit:
N/A


Function:
Its principal effects are increased production and release of corticosteroids and, as its name suggests, cortisol from the adrenal cortex.


Location:
Adrenocorticotropic hormone (ACTH), also known as 'corticotropin', 'Adrenocorticotrophic hormone', is a polypeptide tropic hormone produced and secreted by the anterior pituitary gland. It is an important component of the hypothalamic-pituitary-adrenal axis and is often produced in response to biological stress (along with corticotropin-releasing hormone from the hypothalamus).


Sample Data
Sample Data
Sample Data
My Cart close
Attention close
History close
Clear All
Comparison close
User
My Cart
Attention
History
Comparison
Top
Message
close_message
Product Name: *
Email: *
Message: *
Inform
new Message
Package Quantity Price ($) Subtotal 1 ($)
Subtotal 2: $
triangle
Package Quantity Price ($)
Do you want to be our agent and get a lower discount?
Please contact us:
Tel:027-59234612(+86)
Fax:027-59759960(+86)
Email:sales@eiaab.com