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ASuA (Shorten Name),
Product Name:

Human ASuA/ Anti-Survivin antibody ELISA Kit

 
Cat.#:

E1814h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.78-50ng/mL
Sensitivity:
0.39ng/mL
Specificity:
Natural and recombinant human Anti-Survivin antibody
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E1814h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Anti-Survivin antibody) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Anti-Survivin antibody. The ELISA analytical biochemical technique of the E1814h kit is based on Anti-Survivin antibody antibody-Anti-Survivin antibody antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Anti-Survivin antibody antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Anti-Survivin antibody. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human ASuA ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.2%

Recovery
Recovery was determined by spiking various levels of Anti-Survivin antibody into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

92

86-98

Plasma

94

88-100

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-Survivin antibody and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

83-93%

107-119%

109-117%

100-110%

EDTA plasma(n=5)

103-114%

87-96%

93-103%

100-112%

heparin plasma(n=5)

107-117%

 

102-114%

83-83%

101-113%

 

General Annotation


Sub Unit:
N/A


Function:
Survivin, also called baculoviral inhibitor of apoptosis repeat-containing 5 or BIRC5, is a protein that, in humans, is encoded by the BIRC5 gene. Survivin is a member of the inhibitor of apoptosis (IAP) family. The survivin protein functions to inhibit caspase activation, thereby leading to negative regulation of apoptosis or programmed cell death. This has been shown by disruption of survivin induction pathways leading to increase in apoptosis and decrease in tumour growth. The survivin protein is expressed highly in most human tumours and fetal tissue, but is completely absent in terminally differentiated cells. This fact therefore makes survivin an ideal target for cancer therapy as cancer cells are targeted while normal cells are left alone. Survivin expression is also highly regulated by the cell cycle and is only expressed in the G2-M phase. It is known that survivin localizes to the mitotic spindle by interaction with tubulin during mitosis and may play a contributing role in regulating mitosis. The molecular mechanisms of survivin regulation are still not well understood, but regulation of survivin seems to be linked to the p53 protein. It also is a direct target gene of the Wnt pathway and is upregulated by beta-catenin.


Location:
N/A


This product has not yet been referenced specifically in any publications.
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