Human OXM/ Oxyntomodulin ELISA Kit

Cat.#:

E2215h

Brand:

EIAab^®

Regulatory Status:

RUO

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

0.312-20ng/mL

Sensitivity:

0.08ng/mL

Specificity:

Natural and recombinant human Oxyntomodulin

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

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Human OXM ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations
Sequence / 3D
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Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤11.2%

Recovery

Recovery was determined by spiking various levels of Oxyntomodulin into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	115	109-120
Plasma	117	111-120

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Oxyntomodulin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	83-93%	95-104%	90-100%	108-118%
EDTA plasma(n=5)	105-115%	107-116%	98-108%	115-125%
heparin plasma(n=5)	107-117%	102-112%	90-102%	101-110%

General Annotation

Sub Unit:

N/A

Function:

Oxyntomodulin is a naturally occurring 37-amino acid peptide hormone found in the colon, produced by the oxyntic (fundic) cells of the oxyntic (fundic) mucosa. It has been found to suppress appetite. A recent study has found that it can be used as a weight loss treatment. The mechanism of action of oxyntomodulin is not well understood. It is known to bind both the GLP-1 receptor and the glucagon receptor, but it is not known whether the effects of the hormone are mediated through these receptors or through an unidentified receptor.

Location:

N/A

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