OXM (Shorten Name),
Product Name:
Human OXM/ Oxyntomodulin ELISA Kit
Cat.#:
E2215h
Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.312-20ng/mL
Sensitivity:
0.08ng/mL
Specificity:
Natural and recombinant human Oxyntomodulin
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E2215h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay)
Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Oxyntomodulin) ELISA Kit target analytes in biological samples. The concentration gradients of
the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Oxyntomodulin. The ELISA analytical biochemical
technique of the E2215h kit is based on Oxyntomodulin antibody-Oxyntomodulin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Oxyntomodulin
antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Oxyntomodulin. Appropriate sample types may include undiluted human body fluids
and/or tissue homogenates,
secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤6.2%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤11.2%
Recovery
Recovery was determined by spiking various levels of Oxyntomodulin into serum and plasma.
|
Sample Type |
Average(%) |
Recovery Range(%) |
|
Serum |
115 |
109-120 |
|
Plasma |
117 |
111-120 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Oxyntomodulin and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1：2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
83-93% |
95-104% |
90-100% |
108-118% |
|
EDTA plasma(n=5) |
105-115% |
107-116% |
98-108% |
115-125% |
|
heparin plasma(n=5) |
107-117%
|
102-112% |
90-102% |
101-110% |
General Annotation
Sub Unit:
N/A
Function:
Oxyntomodulin is a naturally occurring 37-amino acid peptide hormone found in the colon, produced by the oxyntic (fundic) cells of the oxyntic (fundic) mucosa. It has been found to suppress appetite. A recent study has found that it can be used as a weight loss treatment.
The mechanism of action of oxyntomodulin is not well understood. It is known to bind both the GLP-1 receptor and the glucagon receptor, but it is not known whether the effects of the hormone are mediated through these receptors or through an unidentified receptor.
Location:
N/A
Vaild Sequence:
This product has not yet been referenced specifically in any publications.
Publishing research using this product ?
Please let us know and we will provide a credit for your next order.
Publishing research using this product ?
Please let us know and we will provide a credit for your next order.
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