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OB (Shorten Name ),
Product Name:

Human OB/ Obestatin ELISA Kit

Cat.#:

E2039h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
15.6-1000pg/mL
Sensitivity:
10pg/mL
Specificity:
Natural and recombinant human Obestatin
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Human OB ELISA Kit
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Human OB ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.8%

Recovery
Recovery was determined by spiking various levels of Obestatin into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

106

100-112

Plasma

108

102-114

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Obestatin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

105-114%

94-106%

106-116%

84-94%

EDTA plasma(n=5)

103-112%

86-95%

103-115%

110-120%

heparin plasma(n=5)

103-113%

 

110-120%

102-112%

90-99%

 

General Annotation


Sub Unit:
N/A


Function:
Obestatin is a putative hormone that is potentially produced in the cells lining the stomach and small intestine of several mammals including humans. Obestatin was originally identified as an anorectic peptide, but its effect on food intake remains controversial.


Location:
N/A


This product has not yet been referenced specifically in any publications.
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