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IP3 (Shorten Name),
Product Name:

General IP3/ Inositol trisphosphate ELISA Kit

 
Cat.#:

E2037Ge

Brand:
EIAab®
Regulatory Status:
Alternative:

Inositol 1,4,5-trisphosphate

Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.625-40ng/mL
Sensitivity:
0.265ng/mL
Specificity:
Natural and recombinant general Inositol trisphosphate
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E2037Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Inositol trisphosphate) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Inositol trisphosphate. The ELISA analytical biochemical technique of the E2037Ge kit is based on Inositol trisphosphate antibody-Inositol trisphosphate antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Inositol trisphosphate antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Inositol trisphosphate. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General IP3 ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Inositol trisphosphate and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

101-111%

107-118%

101-111%

82-91%

EDTA plasma(n=5)

96-107%

106-115%

88-98%

86-97%

heparin plasma(n=5)

107-118%

 

88-98%

107-116%

102-112%

 

General Annotation


Sub Unit:
N/A


Function:
Inositol trisphosphate or inositol 1,4,5-trisphosphate (also commonly known as triphosphoinositol; abbreviated InsP3 or IP3), together with diacylglycerol (DAG), is a secondary messenger molecule used in signal transduction and lipid signaling in biological cells. While DAG stays inside the membrane, IP3 is soluble and diffuses through the cell. It is made by hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2), a phospholipid that is located in the plasma membrane, by phospholipase C (PLC).


Location:
N/A


Sample Data
Sample Data
Sample Data
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