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CML (Shorten Name),
Product Name:

General CML/ N(6)-Carboxymethyllysine ELISA Kit

 
Cat.#:

E1977Ge

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.78-50ng/mL
Sensitivity:
0.25ng/mL
Specificity:
Natural and recombinant general N(6)-Carboxymethyllysine
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E1977Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (N(6)-Carboxymethyllysine) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing N(6)-Carboxymethyllysine. The ELISA analytical biochemical technique of the E1977Ge kit is based on N(6)-Carboxymethyllysine antibody-N(6)-Carboxymethyllysine antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect N(6)-Carboxymethyllysine antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, N(6)-Carboxymethyllysine. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General CML ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of N(6)-Carboxymethyllysine and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

106-116%

90-100%

84-94%

108-118%

EDTA plasma(n=5)

97-107%

98-109%

109-119%

99-111%

heparin plasma(n=5)

100-108%

 

108-117%

80-92%

102-111%

 

General Annotation


Sub Unit:
N/A


Function:
N(6)-Carboxymethyllysine (CML), also known as N(epsilon)-(carboxymethyl)lysine, is an advanced glycation endproduct (AGE). CML has been the most used marker for AGEs in food analysis.


Location:
N/A


This product has not yet been referenced specifically in any publications.
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Sample Data
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