General CML/ N(6)-Carboxymethyllysine ELISA Kit

Cat.#:

E1977Ge

Brand:

EIAab^®

Regulatory Status:

RUO

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

0.78-50ng/mL

Sensitivity:

0.25ng/mL

Specificity:

Natural and recombinant general N(6)-Carboxymethyllysine

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Product Overview:

E1977Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (N(6)-Carboxymethyllysine) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing N(6)-Carboxymethyllysine. The ELISA analytical biochemical technique of the E1977Ge kit is based on N(6)-Carboxymethyllysine antibody-N(6)-Carboxymethyllysine antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect N(6)-Carboxymethyllysine antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, N(6)-Carboxymethyllysine. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

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General CML ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations
Sequence / 3D
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Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of N(6)-Carboxymethyllysine and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	106-116%	90-100%	84-94%	108-118%
EDTA plasma(n=5)	97-107%	98-109%	109-119%	99-111%
heparin plasma(n=5)	100-108%	108-117%	80-92%	102-111%

General Annotation

Sub Unit:

N/A

Function:

N(6)-Carboxymethyllysine (CML), also known as N(epsilon)-(carboxymethyl)lysine, is an advanced glycation endproduct (AGE). CML has been the most used marker for AGEs in food analysis.

Location:

N/A

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