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AGE (Shorten Name),
Product Name:

Human AGE/ Advanced glycation end-product ELISA Kit

 
Cat.#:

E0263h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.78-50ng/mL
Sensitivity:
0.41ng/mL
Specificity:
Natural and recombinant human Advanced glycation end-product
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0263h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Advanced glycation end-product) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Advanced glycation end-product. The ELISA analytical biochemical technique of the E0263h kit is based on Advanced glycation end-product antibody-Advanced glycation end-product antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Advanced glycation end-product antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Advanced glycation end-product. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human AGE ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.6%

Recovery
Recovery was determined by spiking various levels of Advanced glycation end-product into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

93

87-99

Plasma

95

89-101

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Advanced glycation end-product and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

86-95%

104-116%

93-103%

104-114%

EDTA plasma(n=5)

100-110%

101-111%

100-110%

97-108%

heparin plasma(n=5)

96-108%

 

85-97%

102-111%

98-108%

 

General Annotation


Sub Unit:
N/A


Function:
An advanced glycation end-product (AGE) is the result of a chain of chemical reactions after an initial glycation reaction. The intermediate products are known, variously, as Amadori, Schiff base and Maillard products, named after the researchers who first described them. (The literature is inconsistent in applying these terms. For example, Maillard reaction products are sometimes considered intermediates and sometimes end products.) Side products generated in intermediate steps may be oxidizing agents (such as hydrogen peroxide), or not (such as beta amyloid proteins). "Glycosylation" is sometimes used for "glycation" in the literature, usually as 'non-enzymatic glycosylation.'


Location:
N/A


Sample Data
Sample Data
Sample Data
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