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2,3-BPG (Shorten Name),
Product Name:

General 2,3-BPG/ 2,3-Bisphosphoglycerate ELISA Kit

 
Cat.#:

E0983Ge

Brand:
EIAab®
Regulatory Status:
Alternative:

2,3-Bisphosphoglyceric acid,2,3-diphosphoglycerate

Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
31.2-2000nmol/mL
Sensitivity:
9.7nmol/mL
Specificity:
Natural and recombinant general 2,3-Bisphosphoglycerate
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0983Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (2,3-Bisphosphoglycerate) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 2,3-Bisphosphoglycerate. The ELISA analytical biochemical technique of the E0983Ge kit is based on 2,3-Bisphosphoglycerate antibody-2,3-Bisphosphoglycerate antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 2,3-Bisphosphoglycerate antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, 2,3-Bisphosphoglycerate. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General 2,3-BPG ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: 4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: 7.4%

Recovery
Recovery was determined by spiking various levels of 2,3-Bisphosphoglycerate into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

96

94-102

Plasma

97

93-99

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 2,3-Bisphosphoglycerate and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

92-104%

86-95%

92-101%

89-101%

EDTA plasma(n=5)

97-108%

87-100%

81-81%

81-91%

heparin plasma(n=5)

94-104%

 

102-102%

102-112%

101-113%

 

General Annotation


Sub Unit:
N/A


Function:
2,3-Bisphosphoglyceric acid (2,3-Bisphosphoglycerate or 2,3-BPG, also known as 2,3-diphosphoglycerate or 2,3-DPG) is a three-carbon isomer of the glycolytic intermediate 1,3-bisphosphoglyceric acid (1,3-BPG). 2,3-BPG is present in human red blood cells (RBC; erythrocyte) at approximately 5 mmol/L. It binds with greater affinity to deoxygenated hemoglobin (e.g. when the red cell is near respiring tissue) than it does to oxygenated hemoglobin (e.g., in the lungs) due to spatial changes: 2,3-BPG (whose size is estimated at about 9 angstroms) fits in the deoxygenated hemoglobin configuration (11 angstroms), but not as well in the oxygenated (5 angstroms). It interacts with deoxygenated hemoglobin beta subunits by decreasing their affinity for oxygen, so it allosterically promotes the release of the remaining oxygen molecules bound to the hemoglobin, thus enhancing the ability of RBCs to release oxygen near tissues that need it most. 2,3-BPG is thus an allosteric effector.


Location:
N/A


Sample Data
Sample Data
Sample Data
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