General HA/ Hyaluronic acid ELISA Kit

Cat.#:

E0182Ge

Brand:

EIAab^®

Regulatory Status:

RUO

Alternative:

Hyaluronan,Hyaluronate

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

6.25-400ng/mL

Sensitivity:

3.125ng/mL

Specificity:

Natural and recombinant general Hyaluronic acid

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Product Overview:

E0182Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Hyaluronic acid) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Hyaluronic acid. The ELISA analytical biochemical technique of the E0182Ge kit is based on Hyaluronic acid antibody-Hyaluronic acid antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Hyaluronic acid antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Hyaluronic acid. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

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General HA ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations ( 4 )
Sequence / 3D
Feedback Wall (0)

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hyaluronic acid and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	95-105%	88-100%	107-119%	92-102%
EDTA plasma(n=5)	92-100%	103-113%	82-93%	100-109%
heparin plasma(n=5)	113-123%	110-120%	86-97%	84-93%

General Annotation

Sub Unit:

N/A

Function:

Hyaluronan (also called hyaluronic acid or hyaluronate or HA) is an anionic, nonsulfated glycosaminoglycan distributed widely throughout connective, epithelial, and neural tissues. It is unique among glycosaminoglycans in that it is nonsulfated, forms in the plasma membrane instead of the Golgi, and can be very large, with its molecular weight often reaching the millions. One of the chief components of the extracellular matrix, hyaluronan contributes significantly to cell proliferation and migration, and may also be involved in the progression of some malignant tumors. The average 70 kg (154 lbs) person has roughly 15 grams of hyaluronan in the body, one-third of which is turned over (degraded and synthesized) every day. Hyaluronic acid is also a component of the group A streptococcal extracellular capsule, and is believed to play a role in virulence.

Location:

N/A

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