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Home  >  ELISA Kit  >  Human APLN13 ELISA Kit
APLN13 (Shorten Name),
Product Name:

Human APLN13/ Apelin-13 ELISA Kit

 
Cat.#:

E1887h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
78-5000pg/mL
Sensitivity:
33pg/mL
Specificity:
Natural and recombinant human Apelin-13
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E1887h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Apelin-13) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Apelin-13. The ELISA analytical biochemical technique of the E1887h kit is based on Apelin-13 antibody-Apelin-13 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Apelin-13 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Apelin-13. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human APLN13 ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.0%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.5%

Recovery
Recovery was determined by spiking various levels of Apelin-13 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

85

80-91

Plasma

87

81-93

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Apelin-13 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

110-120%

99-107%

103-114%

98-108%

EDTA plasma(n=5)

98-108%

90-99%

98-108%

111-120%

heparin plasma(n=5)

88-97%

 

94-104%

101-113%

109-120%

 

General Annotation


Sub Unit:
N/A


Function:
Apelin gene encodes a pre-proprotein of 77 amino acids, with a signal peptide in the N-terminal region. After translocation into the endoplasmic reticulum and cleavage of the signal peptide, the proprotein of 55 amino acids may generate several active fragments: a 36 amino acid peptide corresponding to the sequence 42-77 (apelin 36), a 17 amino acid peptide corresponding to the sequence 61-77 (apelin 17) and a 13 amino acid peptide corresponding to the sequence 65-77 (apelin 13).


Location:
N/A



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