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APLN13 (Shorten Name ),
Product Name:

Human APLN13/ Apelin-13 ELISA Kit

Cat.#:

E1887h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
78-5000pg/mL
Sensitivity:
33pg/mL
Specificity:
Natural and recombinant human Apelin-13
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Human APLN13 ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.0%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.5%

Recovery
Recovery was determined by spiking various levels of Apelin-13 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

85

80-91

Plasma

87

81-93

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Apelin-13 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

110-120%

99-107%

103-114%

98-108%

EDTA plasma(n=5)

98-108%

90-99%

98-108%

111-120%

heparin plasma(n=5)

88-97%

 

94-104%

101-113%

109-120%

 

General Annotation


Sub Unit:
N/A


Function:
Apelin gene encodes a pre-proprotein of 77 amino acids, with a signal peptide in the N-terminal region. After translocation into the endoplasmic reticulum and cleavage of the signal peptide, the proprotein of 55 amino acids may generate several active fragments: a 36 amino acid peptide corresponding to the sequence 42-77 (apelin 36), a 17 amino acid peptide corresponding to the sequence 61-77 (apelin 17) and a 13 amino acid peptide corresponding to the sequence 65-77 (apelin 13).


Location:
N/A



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