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VLDL (Shorten Name),
Product Name:

Human VLDL/ Very low-density lipoprotein ELISA Kit

 
Cat.#:

E1847h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.312-20ng/mL
Sensitivity:
0.23ng/mL
Specificity:
Natural and recombinant human Very low-density lipoprotein
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E1847h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Very low-density lipoprotein) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Very low-density lipoprotein. The ELISA analytical biochemical technique of the E1847h kit is based on Very low-density lipoprotein antibody-Very low-density lipoprotein antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Very low-density lipoprotein antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Very low-density lipoprotein. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human VLDL ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.6%

Recovery
Recovery was determined by spiking various levels of Very low-density lipoprotein into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

103

97-109

Plasma

105

99-111

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Very low-density lipoprotein and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

102-111%

108-117%

80-80%

98-109%

EDTA plasma(n=5)

81-91%

91-101%

102-111%

108-118%

heparin plasma(n=5)

93-102%

 

100-109%

101-112%

102-111%

 

General Annotation


Sub Unit:
N/A


Function:
Very-low-density lipoprotein (VLDL) is a type of lipoprotein made by the liver. VLDL is one of the five major groups of lipoproteins (chylomicrons, VLDL, low-density lipoprotein, intermediate-density lipoprotein, high-density lipoprotein) that enable fats and cholesterol to move within the water-based solution of the bloodstream. VLDL is assembled in the liver from triglycerides, cholesterol, and apolipoproteins. VLDL is converted in the bloodstream to low-density lipoprotein (LDL). VLDL particles have a diameter of 30-80 nm. VLDL transports endogenous products, whereas chylomicrons transport exogenous (dietary) products.


Location:
N/A



[1].
Zhixiong Pan , Jiwen Wang , Hui Tang , Liang Li , Jia Lv , Chunchun Han , Lu Xia , Feng Xu
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