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首页  >  酶联免疫试剂盒  >  Human Opi ELISA Kit
Opi (短名 ),
产品名称:

Human Opi/ Opiorphin ELISA Kit
Opiorphin

货号:

E1779h

商标:
EIAab®
监管等级:
检测方法:
ELISA
实验类型:
Competitive
检测范围:
0.156-10ng/mL
灵敏度:
0.078ng/mL
特异性:
Natural and recombinant human Opiorphin
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
-
Human Opi ELISA Kit
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产品说明书
数据表: 下载说明书
说明书: 下载说明书
MSDS: MSDS


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤4.9%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤7.8%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Sample Type

Average(%)

Recovery Range(%)

Serum

96

90-102

Plasma

98

92-104

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

108-116%

99-107%

93-102%

96-105%

EDTA plasma(n=5)

104-114%

100-110%

106-116%

98-108%

heparin plasma(n=5)

85-97%

 

102-112%

91-100%

108-118%

 

通用注释


亚单元:
N/A


功能:
Opiorphin is an endogenous chemical compound first isolated from human saliva. Initial research with mice shows the compound has a painkilling effect greater than that of morphine. It works by stopping the normal breakdown of enkephalins, natural pain-killing opioids in the spinal cord. It is a relatively simple molecule consisting of a five-amino acid polypeptide, Gln-Arg-Phe-Ser-Arg. Opiorphin pentapeptide originates from the N-terminal region of the protein PROL1 (proline rich, lacrimal 1). Opiorphin inhibits three proteases: neutral ecto-endopeptidase (MME), ecto-aminopeptidase N (ANPEP) and perhaps also a dipeptidyl peptidase DPP3. Such action extends the duration of enkephalin effect where the natural pain killers are released physiologically in response to specific potentially painful stimuli, in contrast with administration of narcotics, which floods the entire body and causes many undesirable adverse reactions, including addiction liability and constipation. In addition, opiorphin may exert anti-depressive action.


Location:
N/A


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