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Opi (Shorten Name),
Product Name:

Human Opi/ Opiorphin ELISA Kit

 
Cat.#:

E1779h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.156-10ng/mL
Sensitivity:
0.078ng/mL
Specificity:
Natural and recombinant human Opiorphin
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E1779h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Opiorphin) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Opiorphin. The ELISA analytical biochemical technique of the E1779h kit is based on Opiorphin antibody-Opiorphin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Opiorphin antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Opiorphin. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human Opi ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.8%

Recovery
Recovery was determined by spiking various levels of Opiorphin into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

96

90-102

Plasma

98

92-104

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Opiorphin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

108-116%

99-107%

93-102%

96-105%

EDTA plasma(n=5)

104-114%

100-110%

106-116%

98-108%

heparin plasma(n=5)

85-97%

 

102-112%

91-100%

108-118%

 

General Annotation


Sub Unit:
N/A


Function:
Opiorphin is an endogenous chemical compound first isolated from human saliva. Initial research with mice shows the compound has a painkilling effect greater than that of morphine. It works by stopping the normal breakdown of enkephalins, natural pain-killing opioids in the spinal cord. It is a relatively simple molecule consisting of a five-amino acid polypeptide, Gln-Arg-Phe-Ser-Arg. Opiorphin pentapeptide originates from the N-terminal region of the protein PROL1 (proline rich, lacrimal 1). Opiorphin inhibits three proteases: neutral ecto-endopeptidase (MME), ecto-aminopeptidase N (ANPEP) and perhaps also a dipeptidyl peptidase DPP3. Such action extends the duration of enkephalin effect where the natural pain killers are released physiologically in response to specific potentially painful stimuli, in contrast with administration of narcotics, which floods the entire body and causes many undesirable adverse reactions, including addiction liability and constipation. In addition, opiorphin may exert anti-depressive action.


Location:
N/A


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