Human Opi/ Opiorphin ELISA Kit

Cat.#:

E1779h

Brand:

EIAab^®

Regulatory Status:

RUO

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

0.156-10ng/mL

Sensitivity:

0.078ng/mL

Specificity:

Natural and recombinant human Opiorphin

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

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Human Opi ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations
Sequence / 3D
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Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.8%

Recovery

Recovery was determined by spiking various levels of Opiorphin into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	96	90-102
Plasma	98	92-104

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Opiorphin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	108-116%	99-107%	93-102%	96-105%
EDTA plasma(n=5)	104-114%	100-110%	106-116%	98-108%
heparin plasma(n=5)	85-97%	102-112%	91-100%	108-118%

General Annotation

Sub Unit:

N/A

Function:

Opiorphin is an endogenous chemical compound first isolated from human saliva. Initial research with mice shows the compound has a painkilling effect greater than that of morphine. It works by stopping the normal breakdown of enkephalins, natural pain-killing opioids in the spinal cord. It is a relatively simple molecule consisting of a five-amino acid polypeptide, Gln-Arg-Phe-Ser-Arg. Opiorphin pentapeptide originates from the N-terminal region of the protein PROL1 (proline rich, lacrimal 1). Opiorphin inhibits three proteases: neutral ecto-endopeptidase (MME), ecto-aminopeptidase N (ANPEP) and perhaps also a dipeptidyl peptidase DPP3. Such action extends the duration of enkephalin effect where the natural pain killers are released physiologically in response to specific potentially painful stimuli, in contrast with administration of narcotics, which floods the entire body and causes many undesirable adverse reactions, including addiction liability and constipation. In addition, opiorphin may exert anti-depressive action.

Location:

N/A

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