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VB6 (Shorten Name),
Product Name:

General VB6/ Vitamin B6 ELISA Kit

 
Cat.#:

E0916Ge

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
3.12-200nmol/L
Sensitivity:
1.26nmol/L
Specificity:
Natural and recombinant general Vitamin B6
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0916Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Vitamin B6) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Vitamin B6. The ELISA analytical biochemical technique of the E0916Ge kit is based on Vitamin B6 antibody-Vitamin B6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Vitamin B6 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Vitamin B6. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General VB6 ELISA Kit
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Product Datasheets
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: 7.2%

Recovery
Recovery was determined by spiking various levels of Vitamin B6 into serum and plasma.

Recovery was determined by spiking various levels of VB6 into serum and plasma .

Sample Type

Average(%)

Recovery Range(%)

Serum

97

93-102

Plasma

98

96-104

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Vitamin B6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

105-114%

116-116%

99-110%

102-112%

EDTA plasma(n=5)

111-119%

102-111%

107-117%

98-98%

heparin plasma(n=5)

102-113%

 

104-113%

107-117%

95-105%

 

General Annotation


Sub Unit:
N/A


Function:
Vitamin B6 is a water-soluble vitamin and is part of the vitamin B complex group. Several forms of the vitamin are known, but pyridoxal phosphate (PLP) is the active form and is a cofactor in many reactions of amino acid metabolism, including transamination, deamination, and decarboxylation. PLP also is necessary for the enzymatic reaction governing the release of glucose from glycogen.


Location:
N/A


Sample Data
Sample Data
Sample Data
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