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MCAb (Shorten Name),
Product Name:

Human MCAb/ Melanocyte antibody ELISA Kit

 
Cat.#:

E0390h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
15.6-1000U/mL
Sensitivity:
7.80U/mL
Specificity:
Natural and recombinant human Melanocyte antibody
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0390h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Melanocyte antibody) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Melanocyte antibody. The ELISA analytical biochemical technique of the E0390h kit is based on Melanocyte antibody antibody-Melanocyte antibody antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Melanocyte antibody antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Melanocyte antibody. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human MCAb ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.7%

Recovery
Recovery was determined by spiking various levels of Melanocyte antibody into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

97

91-103

Plasma

99

93-105

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Melanocyte antibody and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

114-126%

106-115%

98-107%

89-102%

EDTA plasma(n=5)

84-93%

96-96%

96-105%

85-97%

heparin plasma(n=5)

106-114%

 

93-103%

84-93%

83-92%

 

General Annotation


Sub Unit:
N/A


Function:
Melanocytes are melanin-producing cells located in the bottom layer (the stratum basale) of the skin's epidermis, the middle layer of the eye (the uvea), the inner ear, meninges, bones, and heart. Melanin is a pigment that is responsible primarily for the color of skin.


Location:
N/A


This product has not yet been referenced specifically in any publications.
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