CH (Shorten Name),
Product Name:
General CH/ Cholesterol ELISA Kit
Cat.#:
E1701Ge
Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.156-10nmol/mL
Sensitivity:
0.068nmol/mL
Specificity:
Natural and recombinant general Cholesterol
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E1701Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay)
Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Cholesterol) ELISA Kit target analytes in biological samples. The concentration gradients of
the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Cholesterol. The ELISA analytical biochemical
technique of the E1701Ge kit is based on Cholesterol antibody-Cholesterol antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Cholesterol
antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Cholesterol. Appropriate sample types may include undiluted human body fluids
and/or tissue homogenates,
secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cholesterol and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1：2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
103-113% |
99-111% |
98-108% |
89-98% |
|
EDTA plasma(n=5) |
92-102% |
82-93% |
82-82% |
104-113% |
|
heparin plasma(n=5) |
111-123%
|
94-102% |
107-116% |
106-116% |
General Annotation
Sub Unit:
N/A
Function:
Cholesterol, from the Greek chole- (bile) and stereos (solid) followed by the chemical suffix -ol for an alcohol, is an organic chemical substance classified as a waxy steroid of fat. It is an essential structural component of mammalian cell membranes and is required to establish proper membrane permeability and fluidity.
In addition to its importance within cells, cholesterol is an important component in the hormonal systems of the body for the manufacture of bile acids, steroid hormones, and vitamin D. Cholesterol is the principal sterol synthesized by animals; in vertebrates it is formed predominantly in the liver. Small quantities are synthesized in other cellular organisms (eukaryotes) such as plants and fungi. It is almost completely absent among prokaryotes, i.e. bacteria.
Although cholesterol is important and necessary for human health, high levels of cholesterol in the blood have been linked to damage to arteries and cardiovascular disease.
Location:
N/A
Vaild Sequence:
This product has not yet been referenced specifically in any publications.
Publishing research using this product ?
Please let us know and we will provide a credit for your next order.
Publishing research using this product ?
Please let us know and we will provide a credit for your next order.
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