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LPS (Shorten Name),
Product Name:

General LPS/ Lipopolysaccharide ELISA Kit

 
Cat.#:

E1526Ge

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
7.8-500 pg/mL
Sensitivity:
3.5 pg/mL
Specificity:
Natural and recombinant general Lipopolysaccharide
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E1526Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Lipopolysaccharide) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Lipopolysaccharide. The ELISA analytical biochemical technique of the E1526Ge kit is based on Lipopolysaccharide antibody-Lipopolysaccharide antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Lipopolysaccharide antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Lipopolysaccharide. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General LPS ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 7.5%

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lipopolysaccharide and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

110-122%

108-116%

92-102%

98-108%

EDTA plasma(n=5)

102-111%

100-112%

108-118%

104-114%

heparin plasma(n=5)

89-99%

 

95-107%

85-94%

97-106%

 

General Annotation


Sub Unit:
N/A


Function:
Lipopolysaccharides (LPS), also known as lipoglycans, are large molecules consisting of a lipid and a polysaccharide joined by a covalent bond; they are found in the outer membrane of Gram-negative bacteria, act as endotoxins and elicit strong immune responses in animals.


Location:
N/A


This product has not yet been referenced specifically in any publications.
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