Human MAU/ Microalbuminuria ELISA Kit

Cat.#:

E1459h

Brand:

EIAab^®

Regulatory Status:

RUO

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

12.5-800ng/mL

Sensitivity:

3.12ng/mL

Specificity:

Natural and recombinant human Microalbuminuria

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Product Overview:

E1459h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Microalbuminuria) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Microalbuminuria. The ELISA analytical biochemical technique of the E1459h kit is based on Microalbuminuria antibody-Microalbuminuria antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Microalbuminuria antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Microalbuminuria. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

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Human MAU ELISA Kit

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Product Datasheets

Instruction:

MSDS:

Data
Citations
Sequence / 3D
Feedback Wall (0)

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.0%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤5.4%

Recovery

Recovery was determined by spiking various levels of Microalbuminuria into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	85	80-91
Plasma	87	81-93

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Microalbuminuria and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	85-95%	93-103%	101-111%	106-116%
EDTA plasma(n=5)	91-100%	80-89%	99-109%	104-114%
heparin plasma(n=5)	98-111%	106-116%	105-116%	94-104%

General Annotation

Sub Unit:

N/A

Function:

Microalbuminuria (Urine albumin) occurs when the kidney leaks small amounts of albumin into the urine, in other words, when there is an abnormally high permeability for albumin in the renal glomerulus.

Location:

N/A

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