MAU (Shorten Name),
Product Name:
Human MAU/ Microalbuminuria ELISA Kit
Cat.#:
E1459h
Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
12.5-800ng/mL
Sensitivity:
3.12ng/mL
Specificity:
Natural and recombinant human Microalbuminuria
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E1459h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay)
Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Microalbuminuria) ELISA Kit target analytes in biological samples. The concentration gradients of
the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Microalbuminuria. The ELISA analytical biochemical
technique of the E1459h kit is based on Microalbuminuria antibody-Microalbuminuria antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Microalbuminuria
antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Microalbuminuria. Appropriate sample types may include undiluted human body fluids
and/or tissue homogenates,
secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.0%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤5.4%
Recovery
Recovery was determined by spiking various levels of Microalbuminuria into serum and plasma.
Sample Type | Average(%) | Recovery Range(%) |
Serum | 85 | 80-91 |
Plasma | 87 | 81-93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Microalbuminuria and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1：2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 85-95% | 93-103% | 101-111% | 106-116% |
EDTA plasma(n=5) | 91-100% | 80-89% | 99-109% | 104-114% |
heparin plasma(n=5) | 98-111%
| 106-116% | 105-116% | 94-104% |
General Annotation
Sub Unit:
N/A
Function:
Microalbuminuria (Urine albumin) occurs when the kidney leaks small amounts of albumin into the urine, in other words, when there is an abnormally high permeability for albumin in the renal glomerulus.
Location:
N/A
Vaild Sequence:
This product has not yet been referenced specifically in any publications.
Publishing research using this product ?
Please let us know and we will provide a credit for your next order.
Publishing research using this product ?
Please let us know and we will provide a credit for your next order.
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