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DCP (Shorten Name),

Product Name:

Human DCP/ Des-gamma carboxyprothrombin ELISA Kit

Cat.#:

E2181h

Brand:

EIAab^®

Regulatory Status:

RUO

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.312-20ng/mL

Sensitivity:

0.15ng/mL

Specificity:

Natural and recombinant human Des-gamma carboxyprothrombin

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Product Overview:

E2181h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Des-gamma carboxyprothrombin) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Des-gamma carboxyprothrombin. The ELISA analytical biochemical technique of the E2181h kit is based on Des-gamma carboxyprothrombin antibody-Des-gamma carboxyprothrombin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Des-gamma carboxyprothrombin antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Des-gamma carboxyprothrombin. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

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Human DCP ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations ( 2 )
Sequence / 3D
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Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.1%

Recovery

Recovery was determined by spiking various levels of Des-gamma carboxyprothrombin into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	102	96-108
Plasma	104	98-110

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Des-gamma carboxyprothrombin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	101-114%	97-107%	102-112%	104-113%
EDTA plasma(n=5)	88-98%	93-102%	90-102%	102-112%
heparin plasma(n=5)	90-99%	95-104%	97-107%	92-102%

General Annotation

Sub Unit:

N/A

Function:

Des-gamma carboxyprothrombin (DCP), also known as protein induced by vitamin K absence/antagonist-II (PIVKA-II), is an abnormal form of the coagulation protein, prothrombin. Normally, the prothrombin precursor undergoes post-translational carboxylation (addition of a carboxylic acid group) by gamma-glutamyl carboxylase in the liver prior to secretion into plasma. DCP/PIVKA-II may be detected in people with deficiency of vitamin K (due to poor nutrition or malabsorption) and in those taking warfarin or other medication that inhibits the action of vitamin K.

Location:

N/A

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