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DCP (Shorten Name),
Product Name:

Human DCP/ Des-gamma carboxyprothrombin ELISA Kit

 
Cat.#:

E2181h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.312-20ng/mL
Sensitivity:
0.15ng/mL
Specificity:
Natural and recombinant human Des-gamma carboxyprothrombin
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E2181h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Des-gamma carboxyprothrombin) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Des-gamma carboxyprothrombin. The ELISA analytical biochemical technique of the E2181h kit is based on Des-gamma carboxyprothrombin antibody-Des-gamma carboxyprothrombin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Des-gamma carboxyprothrombin antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Des-gamma carboxyprothrombin. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human DCP ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.1%

Recovery
Recovery was determined by spiking various levels of Des-gamma carboxyprothrombin into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

102

96-108

Plasma

104

98-110

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Des-gamma carboxyprothrombin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

101-114%

97-107%

102-112%

104-113%

EDTA plasma(n=5)

88-98%

93-102%

90-102%

102-112%

heparin plasma(n=5)

90-99%

 

95-104%

97-107%

92-102%

 

General Annotation


Sub Unit:
N/A


Function:
Des-gamma carboxyprothrombin (DCP), also known as protein induced by vitamin K absence/antagonist-II (PIVKA-II), is an abnormal form of the coagulation protein, prothrombin. Normally, the prothrombin precursor undergoes post-translational carboxylation (addition of a carboxylic acid group) by gamma-glutamyl carboxylase in the liver prior to secretion into plasma. DCP/PIVKA-II may be detected in people with deficiency of vitamin K (due to poor nutrition or malabsorption) and in those taking warfarin or other medication that inhibits the action of vitamin K.


Location:
N/A


Sample Data
Sample Data
Sample Data
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